Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions

Mohamed Effat Khalil

doi:doi:10.11648/j.ajls.20221001.11

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Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions

Mohamed Effat Khalil

Published in American Journal of Life Sciences (Volume 10, Issue 1)

Received: 11 December 2021 Accepted: 4 January 2022 Published: 18 January 2022

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Abstract

Infection of cantaloupe plant with Downy mildew caused by the fungus Pseudoperonospora cubensis leads to significant losses in the amount of the crop, in addition to, reducing its marketing value in many countries around the world, therefore, the efficacy of each of the organisms (Trichoderma harzianum, Trichoderma viride, Bacillus subtilis, Bacillus megaterium and Pseudomonas fluorescens), and the fungicide Ridomil gold plus 71.5 WP for the control of cantaloupe downy mildew under laboratory and greenhouse conditions was studied, initially, under laboratory conditions, the results obtained showed that, when using bio filters, in addition to, the recommended concentration of the fungicide to inhibit the germination of P. cubensis sporangia, a significant reduction in the germination rate of the pathogenic fungus sporangia was observed, also, under greenhouse conditions, treatment of cantaloupe with the tested organisms and with the fungicide significantly reduced the severity of downy mildew, compared to the control treatment, where, the best of these treatments in reducing the severity of the disease was the use of the fungicide, while, the organisms were arranged according to their efficiency in reducing the severity of the disease in the following descending order: B. subtilis, Ba. megaterium, P. fluorescens, T. viride and T. harzianum, In addition, cantaloupe plants showed a significant increase in plant growth parameters, for example, (number of leaves, leaf area and total chlorophyll content), also, an increase in yield components, For example, (number of fruits/plant and fruit weight /plant), the above treatments also increased the activity of defense-related enzymes, for example, peroxidase and polyphenol oxidase enzymes, as well as, total phenol content compared to the control treatment.

Published in	American Journal of Life Sciences (Volume 10, Issue 1)
DOI	10.11648/j.ajls.20221001.11
Page(s)	1-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pseudoperonospora cubensis, Biological Organisms, Fungicide, Enzyme Activity

References

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Mohamed Effat Khalil. (2022). Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions. American Journal of Life Sciences, 10(1), 1-9. https://doi.org/10.11648/j.ajls.20221001.11

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Mohamed Effat Khalil. Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions. Am. J. Life Sci. 2022, 10(1), 1-9. doi: 10.11648/j.ajls.20221001.11

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AMA Style

Mohamed Effat Khalil. Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions. Am J Life Sci. 2022;10(1):1-9. doi: 10.11648/j.ajls.20221001.11

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@article{10.11648/j.ajls.20221001.11,
  author = {Mohamed Effat Khalil},
  title = {Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions},
  journal = {American Journal of Life Sciences},
  volume = {10},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.ajls.20221001.11},
  url = {https://doi.org/10.11648/j.ajls.20221001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20221001.11},
  abstract = {Infection of cantaloupe plant with Downy mildew caused by the fungus Pseudoperonospora cubensis leads to significant losses in the amount of the crop, in addition to, reducing its marketing value in many countries around the world, therefore, the efficacy of each of the organisms (Trichoderma harzianum, Trichoderma viride, Bacillus subtilis, Bacillus megaterium and Pseudomonas fluorescens), and the fungicide Ridomil gold plus 71.5 WP for the control of cantaloupe downy mildew under laboratory and greenhouse conditions was studied, initially, under laboratory conditions, the results obtained showed that, when using bio filters, in addition to, the recommended concentration of the fungicide to inhibit the germination of P. cubensis sporangia, a significant reduction in the germination rate of the pathogenic fungus sporangia was observed, also, under greenhouse conditions, treatment of cantaloupe with the tested organisms and with the fungicide significantly reduced the severity of downy mildew, compared to the control treatment, where, the best of these treatments in reducing the severity of the disease was the use of the fungicide, while, the organisms were arranged according to their efficiency in reducing the severity of the disease in the following descending order: B. subtilis, Ba. megaterium, P. fluorescens, T. viride and T. harzianum, In addition, cantaloupe plants showed a significant increase in plant growth parameters, for example, (number of leaves, leaf area and total chlorophyll content), also, an increase in yield components, For example, (number of fruits/plant and fruit weight /plant), the above treatments also increased the activity of defense-related enzymes, for example, peroxidase and polyphenol oxidase enzymes, as well as, total phenol content compared to the control treatment.},
 year = {2022}
}

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TY  - JOUR
T1  - Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions
AU  - Mohamed Effat Khalil
Y1  - 2022/01/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ajls.20221001.11
DO  - 10.11648/j.ajls.20221001.11
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20221001.11
AB  - Infection of cantaloupe plant with Downy mildew caused by the fungus Pseudoperonospora cubensis leads to significant losses in the amount of the crop, in addition to, reducing its marketing value in many countries around the world, therefore, the efficacy of each of the organisms (Trichoderma harzianum, Trichoderma viride, Bacillus subtilis, Bacillus megaterium and Pseudomonas fluorescens), and the fungicide Ridomil gold plus 71.5 WP for the control of cantaloupe downy mildew under laboratory and greenhouse conditions was studied, initially, under laboratory conditions, the results obtained showed that, when using bio filters, in addition to, the recommended concentration of the fungicide to inhibit the germination of P. cubensis sporangia, a significant reduction in the germination rate of the pathogenic fungus sporangia was observed, also, under greenhouse conditions, treatment of cantaloupe with the tested organisms and with the fungicide significantly reduced the severity of downy mildew, compared to the control treatment, where, the best of these treatments in reducing the severity of the disease was the use of the fungicide, while, the organisms were arranged according to their efficiency in reducing the severity of the disease in the following descending order: B. subtilis, Ba. megaterium, P. fluorescens, T. viride and T. harzianum, In addition, cantaloupe plants showed a significant increase in plant growth parameters, for example, (number of leaves, leaf area and total chlorophyll content), also, an increase in yield components, For example, (number of fruits/plant and fruit weight /plant), the above treatments also increased the activity of defense-related enzymes, for example, peroxidase and polyphenol oxidase enzymes, as well as, total phenol content compared to the control treatment.
VL  - 10
IS  - 1
ER  -

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Mohamed Effat Khalil

Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt

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Mohamed Effat Khalil. (2022). Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions. American Journal of Life Sciences, 10(1), 1-9. https://doi.org/10.11648/j.ajls.20221001.11

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Mohamed Effat Khalil. Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions. Am. J. Life Sci. 2022, 10(1), 1-9. doi: 10.11648/j.ajls.20221001.11

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AMA Style

Mohamed Effat Khalil. Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions. Am J Life Sci. 2022;10(1):1-9. doi: 10.11648/j.ajls.20221001.11

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@article{10.11648/j.ajls.20221001.11,
  author = {Mohamed Effat Khalil},
  title = {Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions},
  journal = {American Journal of Life Sciences},
  volume = {10},
  number = {1},
  pages = {1-9},
  doi = {10.11648/j.ajls.20221001.11},
  url = {https://doi.org/10.11648/j.ajls.20221001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20221001.11},
  abstract = {Infection of cantaloupe plant with Downy mildew caused by the fungus Pseudoperonospora cubensis leads to significant losses in the amount of the crop, in addition to, reducing its marketing value in many countries around the world, therefore, the efficacy of each of the organisms (Trichoderma harzianum, Trichoderma viride, Bacillus subtilis, Bacillus megaterium and Pseudomonas fluorescens), and the fungicide Ridomil gold plus 71.5 WP for the control of cantaloupe downy mildew under laboratory and greenhouse conditions was studied, initially, under laboratory conditions, the results obtained showed that, when using bio filters, in addition to, the recommended concentration of the fungicide to inhibit the germination of P. cubensis sporangia, a significant reduction in the germination rate of the pathogenic fungus sporangia was observed, also, under greenhouse conditions, treatment of cantaloupe with the tested organisms and with the fungicide significantly reduced the severity of downy mildew, compared to the control treatment, where, the best of these treatments in reducing the severity of the disease was the use of the fungicide, while, the organisms were arranged according to their efficiency in reducing the severity of the disease in the following descending order: B. subtilis, Ba. megaterium, P. fluorescens, T. viride and T. harzianum, In addition, cantaloupe plants showed a significant increase in plant growth parameters, for example, (number of leaves, leaf area and total chlorophyll content), also, an increase in yield components, For example, (number of fruits/plant and fruit weight /plant), the above treatments also increased the activity of defense-related enzymes, for example, peroxidase and polyphenol oxidase enzymes, as well as, total phenol content compared to the control treatment.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Efficiency of Using Some Biological Organisms as Biological Catalysts to Reduce the Incidence of Cantaloupe Downy Mildew Disease Under Greenhouse Conditions
AU  - Mohamed Effat Khalil
Y1  - 2022/01/18
PY  - 2022
N1  - https://doi.org/10.11648/j.ajls.20221001.11
DO  - 10.11648/j.ajls.20221001.11
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 1
EP  - 9
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20221001.11
AB  - Infection of cantaloupe plant with Downy mildew caused by the fungus Pseudoperonospora cubensis leads to significant losses in the amount of the crop, in addition to, reducing its marketing value in many countries around the world, therefore, the efficacy of each of the organisms (Trichoderma harzianum, Trichoderma viride, Bacillus subtilis, Bacillus megaterium and Pseudomonas fluorescens), and the fungicide Ridomil gold plus 71.5 WP for the control of cantaloupe downy mildew under laboratory and greenhouse conditions was studied, initially, under laboratory conditions, the results obtained showed that, when using bio filters, in addition to, the recommended concentration of the fungicide to inhibit the germination of P. cubensis sporangia, a significant reduction in the germination rate of the pathogenic fungus sporangia was observed, also, under greenhouse conditions, treatment of cantaloupe with the tested organisms and with the fungicide significantly reduced the severity of downy mildew, compared to the control treatment, where, the best of these treatments in reducing the severity of the disease was the use of the fungicide, while, the organisms were arranged according to their efficiency in reducing the severity of the disease in the following descending order: B. subtilis, Ba. megaterium, P. fluorescens, T. viride and T. harzianum, In addition, cantaloupe plants showed a significant increase in plant growth parameters, for example, (number of leaves, leaf area and total chlorophyll content), also, an increase in yield components, For example, (number of fruits/plant and fruit weight /plant), the above treatments also increased the activity of defense-related enzymes, for example, peroxidase and polyphenol oxidase enzymes, as well as, total phenol content compared to the control treatment.
VL  - 10
IS  - 1
ER  -

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