Assessment of <i>Escherichia coli</i> O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Adogo Lillian Yami; Upla Peter Uteh; Ekpiken Solomon Ekpiken

doi:doi:10.11648/j.fem.20180403.12

| Peer-Reviewed

Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Adogo Lillian Yami, Upla Peter Uteh, Ekpiken Solomon Ekpiken

Published in Frontiers in Environmental Microbiology (Volume 4, Issue 3)

Received: 22 May 2018 Accepted: 7 June 2018 Published: 21 July 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Abattoir activities generate numerous waste and microorganisms that have polluting effects on its environment and consequently could have negative impact on the health of its surrounding residents. This study assessed Escherichia coli O157:H7 contamination on adjourning soil and water sources proximal to Abattoirs within Cross River State, South-Eastern Nigeria. A total of 288 composite soils were collected into sterile polyethene bags. A total of 144 water samples were collected in triplicates aseptically into sterile 200 ml plastic bottles. Standard microbiological and biochemical tests were carried out to isolate and identify E. coli O157:H7 from the samples collected. Results showed that surface and subsurface soil samples closest to abattoir slabs had highest prevalence value (22.92%) which decreased significantly (p<0.05) with increase in distance away from the slabs. Spring water sources close to abattoirs had highest prevalence (15.28%) while least value of 3.47% was obtained from boreholes. Prevalence values differed significantly (p<0.05) among the different water sources. Effluents generated from the abattoir causes contamination when introduced to the adjoining soil and water bodies, making them unsuitable for domestic use. The isolation of E. coli O157:H7 strains further demonstrates the need for adequate waste disposal and treatment.

Published in	Frontiers in Environmental Microbiology (Volume 4, Issue 3)
DOI	10.11648/j.fem.20180403.12
Page(s)	88-93
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Escherichia coli, Abattoirs, Effluents, Wastewater, Soil Contamination

References

[1]	O. P. Canencia, M. D. Dalugdug, A. M. Emano, R. Mendoza, and A. M. P. Walag, “Slaughter waste effluents and river and catchment watershed contamination in Caygayan de Oro City, Philippines”. Env. Sci. vol. 9, pp. 142-148, 2016.
[2]	W. D. Nafarnda, I. E., Ajayi, J. C. Shawulu, M. S. Kawe, G. K. Omeiza, N. A. Sani, O. Z. Tenuche, D. D. Dantong, and S. Z. Tags, “Bacteriological quality of abattoir effluents discharged into water bodies in Abuja, Nigeria,” ISRN Vet. Sci., 2012, DOI: 10.5402/2012/515689.
[3]	A. K. B. Amorim, I. R. de Nardiand, and N. V. Del, “Water conservation and effluent minimization: Case study of a poultry slaughterhouse” Resour. Conserv. Recycl., vol. 51, pp. 93–100, 2007.
[4]	C. F. Bustillo-Lecompte, and M. Mehrvar, “Slaughterhouse wastewater characteristics, treatment, and management in the meat processing industry: A review on trends and advances. J. Env. Manag. vol. 161, pp. 287–302, 2015.
[5]	J. Fearon,, S. B. Mensah, and V. Boateng, “Abattoir operations, waste generation and management in the Tamale metropolis: Case study of the Tamale slaughterhouse” J. Pub. Heal. Epi. Vol. 6, pp. 14-19, 2014.
[6]	S. C. Izah, S. E. Bassey, and E. I. Ohimain, “Assessment of Some Selected Heavy Metals in Saccharomyces cerevisiae Biomass Produced from Cassava Mill Effluents,” EC Microbi. Vol. 12, pp. 213-223, 2017.
[7]	A. O. Akinro, O. B. Ikumawoyi, O. Yahaya, and M. M. Ologunagba, “Environmental Impacts of Polyethylene Generation and Disposal in Akure City, Nigeria” Glo. J. Sci. Fron. Res. Agri. Bio., vol. 12, pp. 1 -7. 2012.
[8]	O. A. Adeyemi-Ale,” Impact of abattoir effluent on the physico-chemical parameters of Gbagi stream (Odo-eran), Ibadan, Nigeria,” Ilo. Jour Sci. vol. 1, pp. 100-109, 2004.
[9]	A. O. Adesemoye, B. O. Opere, and S. C. O. Makinde, “Microbial Content of abattoir waste water and its contaminated soil in Lagos, Nigeria,” African Journal of Biotechnology, vol. 5, pp. 1963-1968, 2006.
[10]	D. N. Ogbonna, and T. J. K. Ideriah, Effect of Abattoir wastewater on chemical characteristics of soil and sediment in Southern Nigeria. J. Sci. Res. Rep., vol. 3, pp. 1612-1632, 2014.
[11]	I. T. Aighewi, E. Zimughan, and I. H. Igbinosa, “Concentrated animal wastes as urban land pollutants: a case study of Liarages in some southern Nigeria’s urban agglomerations,” J. Pollut. Eff. Cont., vol 5, pp. 1 -6, 2017.
[12]	M. D. Sobsey, L. A Khatib., V. R. Hill, E. Alocilja, and S. Pillai, “Pathogens in Animal Wastes and the Impacts of Waste Management Practices on their Survival, Transport, and Fate. White paper for The National Centre for Manure and Agricultural Waste Management, 2002, http://www.mwpshq.org/., 2002.
[13]	S. M. Jiban, M. K. Biswas, A. Sagar, D. Suneel, and A. B. Akolkar, (2016). Slaughterhouse in Uttar Pradesh with Hygienic Environment - A Case Study,” Int. J. Res. Appl. Chem, vol. 2, pp. 10–20, 2016.
[14]	I. B. M. Kosamu, J. Mawenda, and H. W. Mapoma, “Water quality changes due to abattoir effluent: A case on Mchesa Stream in Blantyre, Malawi,” Afr. J. Env. Sci. Tech., vol. 5, pp. 589–594, 2011.
[15]	J. O. Ojo, and O. F. Alamuoye, “Bacteriological and physicochemical characteristics of abattoir effluents from Ado - Ekiti Municipal Abattoir Ekiti State, Nigeria,” Sch. Acad. J. Bio. Vol. 3, pp. 838-841, 2015.
[16]	National Bureau of Statistics. (2012). Annual Abstract of Statistics, pp 23, 2012.
[17]	M. Soltan-Dallal, A. Tabarraie, K. MoezArdalan, ”Comparison of four methods for isolation of Yersinia enterolitica from raw and pasteurized milk from Northern Iran,” Int. J. F. Microbiol. vol. 94, pp. 87-91, 2004.
[18]	Milley, D. G, and Sekia, L. H., (1993). An Enzyme-linked Immunosorbent Assay-based isolation procedure for verotoxigenic E. coli. Applied and Environmental Microbiology. 59 (12):4223-4229.
[19]	D. Warburton, D. and D. Christensen. (2006). Isolation of E. coli O157: H7 or NM in foods. MFLP-80. Health Canada. The compendium of analytical methods. Polyscience Publications, Laval, Quebec, Canada.
[20]	N. Pradel, V. Lirelli, C. Champs, J. B. Palcoux, A. Reynaud, F. Cheutz, F. J. Sirot, B. Joly, and C. Forestier,” Prevalence and characterization of Shiga-toxin producing Escherichia coli isolated from cattle, food and children during a one-year prospective study in France,” J. Cli. Microbiol. 38:1023-1031. 2000.
[21]	I. R. Iroha, O. B. Eromonsele, I. B., Moses, F. N., Afiukwa, A. E. Nwakaeze, and P. C. Ejikeugwu, “In vitro antibiogram of multidrug resistant bacteria isolated from Ogbete abattoir effluent in Enugu State, Nigeria,” Int. Res. J. Pub. Env. Heal. vol 3. Pp. 1-6, 2016.
[22]	A. J. Akinyeye, W. T. Aborisade, and A. O. Akinmolayan, “Bacteriological Evaluation of Abattior effluent contaminated soil in Ikpoba, Benin, Nigeria,” Res. Rev. J. Bas. Sci. vol. 1 Article ID 300020. 2013.
[23]	H. A. Neboh, O. A. Ilusanya, C. C. Ezekoye and F. A. Orji, “Assessment of Ijebu-Igbo Abattoir effluent and its impact on the ecology of the receiving soil and river,” IOSR Journal of Environmental Science, Toxicology and Food Technology, vol. 7 pp. 61-67, 2013.
[24]	A. M. Ibekwe, C. M. Grieve, and C. H. Yang, “Survival of E. coli O157:H7 in soil and on lettuce after soil fumigation,” Can. J. Microbio. vol. 53 (5):623- 635. 2007.
[25]	J. O. Nduka, and A. O. Aigberua, “Heavy Metals and Physicochemical Characteristics of soils from the Banks of Effluent Wastewater Retention Pits in the Niger Delta, Nigeria,” Biotechnol Res. Vol. 4, pp. 48-53, 2018.
[26]	O. O. Olayinka, O. H. Adedeji, and I. B. Oladeru,” Water Quality and bacteriological assessment of slaughter house effluent on Urban River in Nigeria,” J. Appl. Sci. Env. Sanit. vol. 8, pp. 277-286, 2013.
[27]	E. I. Seiyaboh, and S. C. Izah, “Bacteriological Assessment of a tidal creek receiving slaughterhouse wastes in Bayelsa State, Nigeria,” Journal of Advances in Biology and Biotechnology, 14 (1): 1-7, 2017.
[28]	H. Dong., S. Lee, W. Kim, J. An, J. Kim, D. Kim, and S. Cho, “Prevalence, virulence potential, and pulse field gel electrophoresis profiling of Shiga toxin-producing Escherichia coli strains from cattle, Gut Pathog. Vol. 9, pp. 1–16, 2017.
[29]	A. A. Diallo, D. Bibbal, F. T. Lo, M. Mbengue, M. M. Sarr, M. Diouf, Y. Sambe, M. Kerouredan, R. Alambedji, Y. Thiongane, E. Oswald, and H. Brugere,” Prevalence of pathogenic and antibiotics resistant Escherichia coli from effluents of a slaughter house and a municipal wastewater treatment plant in Dakar, Afr. Jour. Microbiol. Res. vol. 11, pp. 1035–1042, 2017.

Cite This Article

Plain Text BibTeX RIS

APA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Adogo Lillian Yami, Upla Peter Uteh, Ekpiken Solomon Ekpiken. (2018). Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria. Frontiers in Environmental Microbiology, 4(3), 88-93. https://doi.org/10.11648/j.fem.20180403.12

Copy | Download

ACS Style

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Adogo Lillian Yami; Upla Peter Uteh; Ekpiken Solomon Ekpiken. Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria. Front. Environ. Microbiol. 2018, 4(3), 88-93. doi: 10.11648/j.fem.20180403.12

Copy | Download

AMA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Adogo Lillian Yami, Upla Peter Uteh, Ekpiken Solomon Ekpiken. Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria. Front Environ Microbiol. 2018;4(3):88-93. doi: 10.11648/j.fem.20180403.12

Copy | Download

@article{10.11648/j.fem.20180403.12,
  author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Adogo Lillian Yami and Upla Peter Uteh and Ekpiken Solomon Ekpiken},
  title = {Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria},
  journal = {Frontiers in Environmental Microbiology},
  volume = {4},
  number = {3},
  pages = {88-93},
  doi = {10.11648/j.fem.20180403.12},
  url = {https://doi.org/10.11648/j.fem.20180403.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180403.12},
  abstract = {Abattoir activities generate numerous waste and microorganisms that have polluting effects on its environment and consequently could have negative impact on the health of its surrounding residents. This study assessed Escherichia coli O157:H7 contamination on adjourning soil and water sources proximal to Abattoirs within Cross River State, South-Eastern Nigeria. A total of 288 composite soils were collected into sterile polyethene bags. A total of 144 water samples were collected in triplicates aseptically into sterile 200 ml plastic bottles. Standard microbiological and biochemical tests were carried out to isolate and identify E. coli O157:H7 from the samples collected. Results showed that surface and subsurface soil samples closest to abattoir slabs had highest prevalence value (22.92%) which decreased significantly (pE. coli O157:H7 strains further demonstrates the need for adequate waste disposal and treatment.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria
AU  - Nfongeh Joseph Fuh
AU  - Owoseni Mojisola Christiana
AU  - Adogo Lillian Yami
AU  - Upla Peter Uteh
AU  - Ekpiken Solomon Ekpiken
Y1  - 2018/07/21
PY  - 2018
N1  - https://doi.org/10.11648/j.fem.20180403.12
DO  - 10.11648/j.fem.20180403.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 88
EP  - 93
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20180403.12
AB  - Abattoir activities generate numerous waste and microorganisms that have polluting effects on its environment and consequently could have negative impact on the health of its surrounding residents. This study assessed Escherichia coli O157:H7 contamination on adjourning soil and water sources proximal to Abattoirs within Cross River State, South-Eastern Nigeria. A total of 288 composite soils were collected into sterile polyethene bags. A total of 144 water samples were collected in triplicates aseptically into sterile 200 ml plastic bottles. Standard microbiological and biochemical tests were carried out to isolate and identify E. coli O157:H7 from the samples collected. Results showed that surface and subsurface soil samples closest to abattoir slabs had highest prevalence value (22.92%) which decreased significantly (pE. coli O157:H7 strains further demonstrates the need for adequate waste disposal and treatment.
VL  - 4
IS  - 3
ER  -

Copy | Download

Author Information

Nfongeh Joseph Fuh

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Owoseni Mojisola Christiana

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Adogo Lillian Yami

Department of Biological Sciences, Faculty of Science, Bingham University, Karu, Nigeria
Upla Peter Uteh

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Ekpiken Solomon Ekpiken

Department of Biological Sciences, Faculty of Science, Cross River University of Technology, Calabar, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Adogo Lillian Yami, Upla Peter Uteh, Ekpiken Solomon Ekpiken. (2018). Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria. Frontiers in Environmental Microbiology, 4(3), 88-93. https://doi.org/10.11648/j.fem.20180403.12

Copy | Download

ACS Style

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Adogo Lillian Yami; Upla Peter Uteh; Ekpiken Solomon Ekpiken. Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria. Front. Environ. Microbiol. 2018, 4(3), 88-93. doi: 10.11648/j.fem.20180403.12

Copy | Download

AMA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Adogo Lillian Yami, Upla Peter Uteh, Ekpiken Solomon Ekpiken. Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria. Front Environ Microbiol. 2018;4(3):88-93. doi: 10.11648/j.fem.20180403.12

Copy | Download

@article{10.11648/j.fem.20180403.12,
  author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Adogo Lillian Yami and Upla Peter Uteh and Ekpiken Solomon Ekpiken},
  title = {Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria},
  journal = {Frontiers in Environmental Microbiology},
  volume = {4},
  number = {3},
  pages = {88-93},
  doi = {10.11648/j.fem.20180403.12},
  url = {https://doi.org/10.11648/j.fem.20180403.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20180403.12},
  abstract = {Abattoir activities generate numerous waste and microorganisms that have polluting effects on its environment and consequently could have negative impact on the health of its surrounding residents. This study assessed Escherichia coli O157:H7 contamination on adjourning soil and water sources proximal to Abattoirs within Cross River State, South-Eastern Nigeria. A total of 288 composite soils were collected into sterile polyethene bags. A total of 144 water samples were collected in triplicates aseptically into sterile 200 ml plastic bottles. Standard microbiological and biochemical tests were carried out to isolate and identify E. coli O157:H7 from the samples collected. Results showed that surface and subsurface soil samples closest to abattoir slabs had highest prevalence value (22.92%) which decreased significantly (pE. coli O157:H7 strains further demonstrates the need for adequate waste disposal and treatment.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Assessment of Escherichia coli O157:H7 Contamination in Soil and Water Sources Proximal to Abattoirs Within Cross River State, Nigeria
AU  - Nfongeh Joseph Fuh
AU  - Owoseni Mojisola Christiana
AU  - Adogo Lillian Yami
AU  - Upla Peter Uteh
AU  - Ekpiken Solomon Ekpiken
Y1  - 2018/07/21
PY  - 2018
N1  - https://doi.org/10.11648/j.fem.20180403.12
DO  - 10.11648/j.fem.20180403.12
T2  - Frontiers in Environmental Microbiology
JF  - Frontiers in Environmental Microbiology
JO  - Frontiers in Environmental Microbiology
SP  - 88
EP  - 93
PB  - Science Publishing Group
SN  - 2469-8067
UR  - https://doi.org/10.11648/j.fem.20180403.12
AB  - Abattoir activities generate numerous waste and microorganisms that have polluting effects on its environment and consequently could have negative impact on the health of its surrounding residents. This study assessed Escherichia coli O157:H7 contamination on adjourning soil and water sources proximal to Abattoirs within Cross River State, South-Eastern Nigeria. A total of 288 composite soils were collected into sterile polyethene bags. A total of 144 water samples were collected in triplicates aseptically into sterile 200 ml plastic bottles. Standard microbiological and biochemical tests were carried out to isolate and identify E. coli O157:H7 from the samples collected. Results showed that surface and subsurface soil samples closest to abattoir slabs had highest prevalence value (22.92%) which decreased significantly (pE. coli O157:H7 strains further demonstrates the need for adequate waste disposal and treatment.
VL  - 4
IS  - 3
ER  -

Copy | Download