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Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities

Received: 9 August 2022     Accepted: 26 August 2022     Published: 5 September 2022
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Abstract

Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.

Published in Frontiers in Environmental Microbiology (Volume 8, Issue 3)
DOI 10.11648/j.fem.20220803.12
Page(s) 55-61
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Surface Water, Escherichia Coli, Esbl Type Ctx-M, Antibiotics, Risk Factors

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Cite This Article
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    Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, et al. (2022). Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Frontiers in Environmental Microbiology, 8(3), 55-61. https://doi.org/10.11648/j.fem.20220803.12

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    ACS Style

    Cecile Ingrid Djuikoue; Farid Wega; Audrey Limou Dayomo; Cedric Dylan Seugnou Nana; Dorine Ngatcheu Ekeu, et al. Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Front. Environ. Microbiol. 2022, 8(3), 55-61. doi: 10.11648/j.fem.20220803.12

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    AMA Style

    Cecile Ingrid Djuikoue, Farid Wega, Audrey Limou Dayomo, Cedric Dylan Seugnou Nana, Dorine Ngatcheu Ekeu, et al. Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities. Front Environ Microbiol. 2022;8(3):55-61. doi: 10.11648/j.fem.20220803.12

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  • @article{10.11648/j.fem.20220803.12,
      author = {Cecile Ingrid Djuikoue and Farid Wega and Audrey Limou Dayomo and Cedric Dylan Seugnou Nana and Dorine Ngatcheu Ekeu and Celianthe Guegang Guegang and Dana Djouela Djoulako and Kensese Sontin Mossanda and Venant Tchokonte Nana and Omer Njajou},
      title = {Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities},
      journal = {Frontiers in Environmental Microbiology},
      volume = {8},
      number = {3},
      pages = {55-61},
      doi = {10.11648/j.fem.20220803.12},
      url = {https://doi.org/10.11648/j.fem.20220803.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220803.12},
      abstract = {Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Freshwater Linked Resistance Profile and Prevalence of Escherichia Coli Producing ESBL Type CTX-M Strains in Cameroon Urban Cities
    AU  - Cecile Ingrid Djuikoue
    AU  - Farid Wega
    AU  - Audrey Limou Dayomo
    AU  - Cedric Dylan Seugnou Nana
    AU  - Dorine Ngatcheu Ekeu
    AU  - Celianthe Guegang Guegang
    AU  - Dana Djouela Djoulako
    AU  - Kensese Sontin Mossanda
    AU  - Venant Tchokonte Nana
    AU  - Omer Njajou
    Y1  - 2022/09/05
    PY  - 2022
    N1  - https://doi.org/10.11648/j.fem.20220803.12
    DO  - 10.11648/j.fem.20220803.12
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 55
    EP  - 61
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20220803.12
    AB  - Mutualistic human digestive bacteria resistant to antibiotics released into the environment have negative effects on health. The aim of this study was to describe the presence of Escherichia coli (E. coli) strains producing extended spectrum β-lactamases of the CTX-M type strains in three urban cities of Cameroon. A total of 79 E. coli strains resistant to cefotaxime were isolated from 200 freshwater samples collected and inoculated in EMB medium supplemented with cefotaxime. Isolated colonies were subjected to an antibiogram according to CA-SFM 2019 and the risk factors associated with their prevalence were also assessed. The results showed that 51% of samples were positive for E. coli CTX-M, a large portion of which (77.4%) produced an extended spectrum β-lactamases-CTX-M type. Meanwhile, only 59% of strains were resistant to β-lactamases. A co-resistance to cotrimoxazole (81%), aminoglycosides (57.3%), quinolones (43%), and fluoroquinolones (17.7%) has been reported. Interestingly, E. coli resistant to cefotaxime strains was influenced by the points of collection, latrines proximity and human activities. A multivariate analysis indicated 39.5% of E. coli ESBL CTX-M strains prevalence associated with proximity to latrines and human activities. The Increasing E. coli ESBL CTX-M strains prevalence in Cameroon is indeed a public health concern. Therefore, a “One Health Approach Operationalization Program” that would include sensitizing the population and improving sanitation interventions in effective waste management, is crucial in maintaining a healthy environment.
    VL  - 8
    IS  - 3
    ER  - 

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Author Information
  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Sciences, University of Douala, Douala, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Faculty of Health Sciences, University des Montagnes, Bangangte, Cameroon

  • Foundation Prevention and Control, Nde Division Prefecture, Bangangte, Cameroon

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