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Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity

Received: 13 March 2023     Accepted: 1 April 2023     Published: 27 April 2023
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Abstract

Phosphate-solubilizing bacteria can enhance the dissolution of insoluble phosphorus in the soil, promoting the availability of soluble phosphorus. Thus, their application can reduce the use of chemical fertilizers and ease sustainable agriculture. The study aimed at isolating and assessing the phosphate solubilizing activity of peanut rhizobia isolated from root nodules of peanut in three Cameroonian soils (Douala, Bafoussam and Ebolowa). Rhizobia were trapped by seeding peanut in plastic pots containing different soils. 45 days later, at the flowering time, root nodules were harvested. From these nodules, rhizobia were isolated, purified on Yeast Extract Mannitol Agar (YEMA) medium and authenticated by inoculating them on sterile sand containing peanut plants and watered with a nutrient solution without nitrogen. The phosphate solubilizing activity of the rhizobia isolates was then assessed in Modified Mineral Salt Medium (MMSM) containing bromocresol green and where the soluble phosphate was replaced by insoluble inorganic phosphates from Cameroon, Algeria, Senegal and tricalcium phosphate in both solid and broth media. The results were analysed statistically by one-way ANOVA using SPSS. A collection of twenty-five isolates was constituted among which 04 from Douala, 12 from Bafoussam and 09 from Ebolowa. All isolates were distinct morphologically. There was significant solubilization of inorganic phosphate in both solid and broth media (p<0.05). Isolated bacteria were characterized as being phosphate solubilizers with values ranging from 1.75 (AhBf1 on Algeria rock phosphate) to 18.9 mg/L (AhDa3 on Algeria rock phosphate). The Algeria rock phosphate was the most solubilized by peanut nodulating bacteria, followed by the tricalcium phosphate, the Senegal rock phosphate and the Cameroon rock phosphate. The solubilizing activity of inorganic phosphates in the broth medium was associated with a global decrease in the pH of the culture medium. The peanut nodulating rhizobia isolates that show the best solubilizing capacity could alleviate the problem of phosphorus availability in agricultural soils.

Published in Frontiers in Environmental Microbiology (Volume 9, Issue 1)
DOI 10.11648/j.fem.20230901.11
Page(s) 1-8
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Peanut, Rhizobium, Phosphate Solubilizing Rhizobia, Cameroon

References
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[3] Elser JJ. Phosphorus: a limiting nutrient for humanity? 2012. Curr Opin Biotechnol. 23 (6): 833-838.
[4] Goldstein AH. 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. Am J Alternative Agr.; 1 (2): 51-57.
[5] Singh, H., and Reddy, M. S. 2011. Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. Eur. J. Soil Biol. 47, 30–34. doi: 10.1016/j.ejsobi.2010.10.005.
[6] Vincent, J. M. 1970. A manual for the practical study of the root nodule bacteria. IBP. Handbook n°15 – Blackwell scientific publishers, Oxford. Edingbourgh. p. 104.
[7] Jordan, D. C. 1984. Family III. Rhizobiaceae Conn. 1938. In: Bergey’s manual of systematic bacteriology. Krieg, N. R. and Holt, J. G. (eds) 1 pp. 234-244.
[8] Reyes, F. et Rodriguez, H. 1999. phosphate solubilizing bacteria and their role in plant growth promotion. Biotech. Adv. 17: 319-339.
[9] Gadagi, R. S., Sa, T. 2002. New isolation method for microorganisms solubilizing iron and aluminium phosphates using dyes. Soil Science and Plant Nutrition. 48: 615-618.
[10] Berraquerro, F. R., Baya, A. M. and Cormenzana, A. R. 1976. Establishment of indices for the study of phosphate solubilzing soil bacteria Ars pharmaceutica. 17: 399-406.
[11] Murphy J, and Riley JP. 1962. A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta 27, 31-36. ws in Plant Sciences 22, 107-149.
[12] Zhang, W. T., Yang, J. K., Yuan, T. Y., Zhou, J. C. 2007. Genetic diversity and phylogeny of indigenous rhizobia from cowpea (Vigna unguiculata (L.) Walp.). Biology and Fertility of Soil, 44 (1): 201-210.
[13] Youagang G. H. S., Ngo Nkot L., Asseng C. C., Nyaka Ngolisa A. I. C., Ngakou A. et Nwaga D. 2020. Isolation and characterization of Legume Nodulating Bacteria Isolated from Common bean (Phaseolus vulgaris L.) nodules. Global Scientific Journals. 8 (5): 1777-1792. Online: ISSN 2320-9186.
[14] Marra, L. M., Oliveira, S. L., Soares, C. R. F. S., Moreira, F. M. S. 2011. Solubilization of inorganic phosphates by strains from tropical legumes. Sci. Agric. 65: 603-609.
[15] Otieno N, Lally RD, Kiwanuka S, Lloyd A, Ryan D, Germaine KJ, Dowling DN. 2015. Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Frontiers in Microbiology 6: 745.
[16] Lin, T. F., Huang, F. T., Youg, C. C., 2006. The proton of gluconic acid are major factor responsible for the dissolution of tricalcium phosphate by Burkolderia cepacia CC- A174. Bioresource Technology 97: 957-960.
[17] Adnane B, Elhaissoufi W, Said K, Benmrid B, Borden K, Rchiad Z. 2021. Benefits of phosphate solubilizing bacteria on belowground crop performance for improved crop acquisition of phosphorus. Microbiol Res. 252: 126842.
[18] Antoun H., and Kloepper, J. W. 2001. Plant Growth promoting rhizobacteria. Encyclopedia of Genetics. Brenner, S. and Miller, J. F. Academic Press. 1477-1480.
[19] Peix, A. Rivas-Boyero., A. A. Mateos., P. F. Rodriguez-Barrueco., C. Martínez-Molina., E. Velazquez, E. 2001. Soil Biology & Biochemistry. 33: 103-110.
[20] Maliha R., S. Khalil, N. Ayub, S. Alam and F. Latif. 2004. Pak. J. Biol. Sciences. 7 (2) 187-196.
Cite This Article
  • APA Style

    Mba Edou Simon Jeremie, Ngo Nkot Laurette, Youagang Gougueu Harris Stephane, Semboung Lang Firmin, Nyaka Ngobissa Aurelie Irene Claire, et al. (2023). Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity. Frontiers in Environmental Microbiology, 9(1), 1-8. https://doi.org/10.11648/j.fem.20230901.11

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    ACS Style

    Mba Edou Simon Jeremie; Ngo Nkot Laurette; Youagang Gougueu Harris Stephane; Semboung Lang Firmin; Nyaka Ngobissa Aurelie Irene Claire, et al. Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity. Front. Environ. Microbiol. 2023, 9(1), 1-8. doi: 10.11648/j.fem.20230901.11

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    AMA Style

    Mba Edou Simon Jeremie, Ngo Nkot Laurette, Youagang Gougueu Harris Stephane, Semboung Lang Firmin, Nyaka Ngobissa Aurelie Irene Claire, et al. Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity. Front Environ Microbiol. 2023;9(1):1-8. doi: 10.11648/j.fem.20230901.11

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  • @article{10.11648/j.fem.20230901.11,
      author = {Mba Edou Simon Jeremie and Ngo Nkot Laurette and Youagang Gougueu Harris Stephane and Semboung Lang Firmin and Nyaka Ngobissa Aurelie Irene Claire and Timb Sara Augustine Laurence and Asseng Charles Carnot},
      title = {Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity},
      journal = {Frontiers in Environmental Microbiology},
      volume = {9},
      number = {1},
      pages = {1-8},
      doi = {10.11648/j.fem.20230901.11},
      url = {https://doi.org/10.11648/j.fem.20230901.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20230901.11},
      abstract = {Phosphate-solubilizing bacteria can enhance the dissolution of insoluble phosphorus in the soil, promoting the availability of soluble phosphorus. Thus, their application can reduce the use of chemical fertilizers and ease sustainable agriculture. The study aimed at isolating and assessing the phosphate solubilizing activity of peanut rhizobia isolated from root nodules of peanut in three Cameroonian soils (Douala, Bafoussam and Ebolowa). Rhizobia were trapped by seeding peanut in plastic pots containing different soils. 45 days later, at the flowering time, root nodules were harvested. From these nodules, rhizobia were isolated, purified on Yeast Extract Mannitol Agar (YEMA) medium and authenticated by inoculating them on sterile sand containing peanut plants and watered with a nutrient solution without nitrogen. The phosphate solubilizing activity of the rhizobia isolates was then assessed in Modified Mineral Salt Medium (MMSM) containing bromocresol green and where the soluble phosphate was replaced by insoluble inorganic phosphates from Cameroon, Algeria, Senegal and tricalcium phosphate in both solid and broth media. The results were analysed statistically by one-way ANOVA using SPSS. A collection of twenty-five isolates was constituted among which 04 from Douala, 12 from Bafoussam and 09 from Ebolowa. All isolates were distinct morphologically. There was significant solubilization of inorganic phosphate in both solid and broth media (p<0.05). Isolated bacteria were characterized as being phosphate solubilizers with values ranging from 1.75 (AhBf1 on Algeria rock phosphate) to 18.9 mg/L (AhDa3 on Algeria rock phosphate). The Algeria rock phosphate was the most solubilized by peanut nodulating bacteria, followed by the tricalcium phosphate, the Senegal rock phosphate and the Cameroon rock phosphate. The solubilizing activity of inorganic phosphates in the broth medium was associated with a global decrease in the pH of the culture medium. The peanut nodulating rhizobia isolates that show the best solubilizing capacity could alleviate the problem of phosphorus availability in agricultural soils.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity
    AU  - Mba Edou Simon Jeremie
    AU  - Ngo Nkot Laurette
    AU  - Youagang Gougueu Harris Stephane
    AU  - Semboung Lang Firmin
    AU  - Nyaka Ngobissa Aurelie Irene Claire
    AU  - Timb Sara Augustine Laurence
    AU  - Asseng Charles Carnot
    Y1  - 2023/04/27
    PY  - 2023
    N1  - https://doi.org/10.11648/j.fem.20230901.11
    DO  - 10.11648/j.fem.20230901.11
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 1
    EP  - 8
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20230901.11
    AB  - Phosphate-solubilizing bacteria can enhance the dissolution of insoluble phosphorus in the soil, promoting the availability of soluble phosphorus. Thus, their application can reduce the use of chemical fertilizers and ease sustainable agriculture. The study aimed at isolating and assessing the phosphate solubilizing activity of peanut rhizobia isolated from root nodules of peanut in three Cameroonian soils (Douala, Bafoussam and Ebolowa). Rhizobia were trapped by seeding peanut in plastic pots containing different soils. 45 days later, at the flowering time, root nodules were harvested. From these nodules, rhizobia were isolated, purified on Yeast Extract Mannitol Agar (YEMA) medium and authenticated by inoculating them on sterile sand containing peanut plants and watered with a nutrient solution without nitrogen. The phosphate solubilizing activity of the rhizobia isolates was then assessed in Modified Mineral Salt Medium (MMSM) containing bromocresol green and where the soluble phosphate was replaced by insoluble inorganic phosphates from Cameroon, Algeria, Senegal and tricalcium phosphate in both solid and broth media. The results were analysed statistically by one-way ANOVA using SPSS. A collection of twenty-five isolates was constituted among which 04 from Douala, 12 from Bafoussam and 09 from Ebolowa. All isolates were distinct morphologically. There was significant solubilization of inorganic phosphate in both solid and broth media (p<0.05). Isolated bacteria were characterized as being phosphate solubilizers with values ranging from 1.75 (AhBf1 on Algeria rock phosphate) to 18.9 mg/L (AhDa3 on Algeria rock phosphate). The Algeria rock phosphate was the most solubilized by peanut nodulating bacteria, followed by the tricalcium phosphate, the Senegal rock phosphate and the Cameroon rock phosphate. The solubilizing activity of inorganic phosphates in the broth medium was associated with a global decrease in the pH of the culture medium. The peanut nodulating rhizobia isolates that show the best solubilizing capacity could alleviate the problem of phosphorus availability in agricultural soils.
    VL  - 9
    IS  - 1
    ER  - 

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Author Information
  • Department of Plant Biology, Faculty of Science, The University of Douala, Douala, Cameroon

  • Department of Plant Biology, Faculty of Science, The University of Douala, Douala, Cameroon

  • Department of Plant Biology, Faculty of Science, The University of Douala, Douala, Cameroon

  • Department of Plant Biology, Faculty of Science, The University of Douala, Douala, Cameroon

  • Institute of Agricultural Research for Development, Njombe, Cameroon

  • Department of Microbiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon

  • Department of Plant Biology, Faculty of Science, The University of Douala, Douala, Cameroon

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