Cowpea leaves are enjoyed as vegetables in many parts of Africa as they contain a lot of antioxidants, micronutrients and nutraceuticals whose deficiency is prevalent among people in Sub-Saharan Africa. Cowpea leaves undergo several physiological and metabolic changes during their maturity stages which may affect their nutritional content. However, farmers lack knowledge on the best cowpea harvesting stage. This research therefore aimed at obtaining information on the right harvesting stage that would enhance cowpea utilization by farmers. Cowpeas variety M66 was planted in RCBD and the treatments which were replicated thrice included harvesting at 21, 35 and 49 DAS. Data was collected on chlorophyll content, iron, calcium, crude fibre, beta carotene, protein and moisture content. The data was subjected for variance using Statistical Analysis System 9.2 edition and significantly different means separated using LSD at 5%. The harvesting stage significantly (p ≤ 0.05) influenced the chlorophyll content with 49 DAS recording the highest content at 51.39 nm followed by 35 DAS with 41.87 nm and the least at 21 DAS with 22. 05 nm. The moisture content decreased with the stage of harvest with highest moisture content being observed at 21 DAS and the least at 49 DAS in both trials. The iron content of cowpea leaves was significantly (p ≤ 0.05) different at 49 DAS in both trials. The calcium content at 21 and 49 DAS in both trials was significantly (p ≤ 0.05) different. The protein content was significant (p ≤ 0.05) in all the stages of harvesting with the highest protein content in both trials being recorded at 21 DAS and the least being recorded at 49 DAS in both. Crude fibre content increased with the stage of harvesting in both trials. This research highlights the essence of harvesting cowpea leaves at the correct harvest stage for increased nutrient utilization.
| Published in | Plant (Volume 11, Issue 2) |
| DOI | 10.11648/j.plant.20231102.12 |
| Page(s) | 50-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Vegetables, Cowpeas, Leaves, Harvesting Stage, Nutrient Content
| [1] | Horn, L., & Shimelis, H. (2020). Production constraints and breeding approaches for cowpea improvement for drought prone agro-ecologies in Sub-Saharan Africa. Annals of Agricultural Sciences, 65 (1), 83-91. |
| [2] | Rashid, A., Mir, M., & Hakeem, K. (2016). Biofertilizer use for sustainable agricultural production. In Plant, Soil and Microbes (pp. 163-180). Springer, Cham. |
| [3] | Kosgey, G. (2021). Nutritional Quality and Anti-Oxidant Properties of Minimally Processed African Indigenous Leafy Vegetables (Doctoral dissertation, JKUAT-Agriculture. |
| [4] | Owade, J., Abong’, G., Okoth, M., & Mwang’ombe, A. (2020). A review of the contribution of cowpea leaves to food and nutrition security in East Africa. Food Science & Nutrition, 8 (1), 36-47. |
| [5] | Katoch, R. (2022). Forage Legumes in Himalayan Region. In Nutritional Quality Management of Forages in the Himalayan Region (pp. 309-353). Springer, Singapore. |
| [6] | Khader V., & Rama, S. (2003). Selected mineral content of common leafy vegetables consumed in India at different stages of maturity. Plant Foods Hum Nutr. 1998; 53 (1): 71-81. |
| [7] | Bergquist, S. (2006). Bioactive compounds in baby spinach (Spinacia oleracea L.): Effects of Pre- and Postharvest factors. Ph. D. Thesis, Swedish University of Agricultural Sciences, Alnarp. |
| [8] | Bayata, A. (2019). Review on nutritional value of cassava for use as a staple food. Sci J Anal Chem, 7 (4), 83-91. |
| [9] | Mijena, D., Mekete, B., & Getiso, A. (2022). Effect of nps fertilizer and harvesting stage on biomass yield and quality parameters of bracharia grass under supplementary irrigation in Southern Ethiopia. Ukrainian Journal of Ecology, 12 (10). |
| [10] | Owino, W. (2022). Effect of harvest stage and nitrogen fertilization on the postharvest shelf life of black nightshade (Solanum nigrum L.) and collard (Brassica oleracea var. acephala L.). African Journal of Food, Agriculture, Nutrition & Development, 22 (6). |
| [11] | Nawab, J., Ghani, J., Khan, S., Khan, M. A., Ali, A., Rahman, Z., & Lei, M. (2022). Nutrient Uptake and Plant Growth Under the Influence of Toxic Elements. In: Sustainable Plant Nutrition under Contaminated Environments (pp. 75-101). Springer, Cham. |
| [12] | Adegbaju, O., Otunola, G., & Afolayan, A. (2019). Proximate, mineral, vitamin and anti-nutrient content of Celosia argentea at three stages of maturity. South African Journal of Botany, 124, 372-379. |
| [13] | Jaetzold, R., Schmidt, H., Hornetz, B., & Shisanya, C. (2006). Farm management handbook of Kenya Vol. II: natural conditions and farm management information Part C East Kenya Subpart C1 Eastern Province. |
| [14] | Nderi, L. (2020). Effect of different spacing intervals on growth and yield of cowpea varieties in Kilifi County, Kenya (Doctoral dissertation, KeMU). |
| [15] | Cerovic, Z., Masdoumier, G., Ghozlen, N., & Latouche, G. (2012). A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids. Physiologia plantarum, 146 (3), 251-260. |
| [16] | AOAC International (2000). Official methods of Analytical Chemistry Analysis of AOAC International 11 th Ed. Method 970.30. The Association: Arlington, VA. |
| [17] | AOAC International (1995). Official methods of Analytical Chemistry Analysis of AOAC International 18 th Ed. Method 999.11. |
| [18] | AOAC International (1995). Official methods of Analytical Chemistry Analysis of AOAC International 17th Ed. Gaithersburg, MD Method 991.20. |
| [19] | AOAC International (2000). Official methods of Analytical Chemistry Analysis of AOAC International 17th Ed. Methods 984.04. The Association: Arlington, VA. |
| [20] | Stessman, D., Miller, A., Spalding, M., & Rodermel, S. (2002). Regulation of photosynthesis during Arabidopsis leaf development in continuous light. Photosynthesis Research, 72, 27-37. |
| [21] | Oguchi, R., Hikosaka, K., & Hirose, T. (2005). Leaf anatomy as a constraint for photosynthetic acclimation: differential responses in leaf anatomy to increasing growth irradiance among three deciduous trees. Plant, Cell & Environment, 28 (7), 916-927. |
| [22] | Budiarto, R., Poerwanto, R., Santosa, E., Efendi, D., & Agusta, A. (2022). Comparative and Correlation Analysis of Young and Mature Kaffir Lime (Citrus Hystrix DC) Leaf Characteristics. International Journal of Plant Biology, 13 (3), 270-280. |
| [23] | Jin, T., Tang, J., Lyu, H., Wang, L., Gillmore, A., & Schaeffer, S. (2022). Activities of Microplastics (MPs) in Agricultural Soil: A Review of MPs Pollution from the Perspective of Agricultural Ecosystems. Journal of Agricultural and Food Chemistry, 70 (14), 4182-4201. |
| [24] | Sanchez, R., Hall, A., Trapani, N., & De Hunau, R. (1983). Effects of water stress on the chlorophyll content, nitrogen level and photosynthesis of leaves of two maize genotypes. Photosynthesis research, 4, 35-47. |
| [25] | Gholamin, R., & Khayatnezhad, M. (2020). Assessment of the correlation between chlorophyll content and drought resistance in corn cultivars (Zea Mays). Helix-The Scientific Explorer | Peer Reviewed Bimonthly International Journal, 10 (05), 93-97. |
| [26] | Hetherington, S., Smillie, R., & Davies, W. (1998). Photosynthetic activities of vegetative and fruiting tissues of tomato. Journal of experimental botany, 49 (324), 1173-1181. |
| [27] | Hokmalipour, S., & Darbandi, M. (2011). Effects of nitrogen fertilizer on chlorophyll content and other leaf indicate in three cultivars of maize (Zea mays L.). World applied sciences journal, 15 (12), 1780-1785. |
| [28] | Bielczynski, L., Łącki, M., Hoefnagels, I., Gambin, A., & Croce, R. (2017). Leaf and plant age affects photosynthetic performance and photoprotective capacity. Plant Physiology, 175 (4), 1634-1648. |
| [29] | Oduntan, A., & Olaleye, O., & Akinwande, B. (2012). Effect of Plant Maturity on the Proximate Composition of Sesamum radiatum Schum Leaves. Journal of Food Studies. 1. 10.5296/jfs.v1i1.1806. |
| [30] | Getahun, E., Gabbiye, N., Delele, M., Fanta, S., Gebrehiwot, M., & Vanierschot, M. (2020). Effect of maturity on the moisture sorption isotherm of chili pepper (Mareko Fana variety). Heliyon, 6 (8), e04608. |
| [31] | Mafokoane, A. (2019). Effect of time-based oven-drying on the nutritional quality of cowpea (Vigna unguiculata) leaves (Doctoral dissertation). |
| [32] | Trivellini, A., Ferrante, A., Vernieri, P., Carmassi, G., & Serra, G. (2011). Spatial and temporal distribution of mineral nutrients and sugars throughout the lifespan of Hibiscus rosa-sinensis L. flower. Central European Journal of Biology 6: 365–375. |
| [33] | Abugre, C. (2011). Assessment of some traditional leafy vegetables of upper east region and influence of stage of harvest and drying method on nutrients content of spider flower (Cleome gynandra L.) (Doctoral dissertation). |
| [34] | Tiffin, L. O. 1972. Translocation of micronutrients in plants. In Micronutrients in Agriculture, Proceedings of a symposium held at Muscle Shoals, Alabama USA, April 20–22, 1971 (J. J. Mortvedt, P. M. Giordano, W. L. Lindsay, eds.) pp. 199– 229, Soil Science Society of America, Inc., Madison, WI. |
| [35] | Flyman, M. V., & Afolayan, A. J. (2008). Effect of plant maturity on the mineral content of the leaves of momordica balsamina l. And vigna unguiculata subsp. Sesquipedalis (l.) Verdc. Journal of Food Quality, 31 (5), 661-671. |
| [36] | Adebiyi, A., & Oluwalana, A. (2019). Effect of Developmental Stages on the Proximate and Mineral Composition of Pleurotus sajor-caju. |
| [37] | Cataldo, D., & Wildung, R. (1978). Soil and plant factors influencing the accumulation of heavy metals by plants. Environmental health perspectives, 27, 149-159. |
| [38] | Modi, M., Modi, A., & Hendriks, S. (2006). Potential role for wild vegetables in household food security: a preliminary case study in Kwazulu-Natal, South Africa. African Journal of Food, Agriculture, Nutrition and Development, 6 (1), 1-13. |
| [39] | Mamboleo, T., Msuya, J., & Mwanri, A. (2018). Vitamin C, iron and zinc levels of selected African green leafy vegetables at different stages of maturity. African Journal of Biotechnology, 17 (17), 567-73. |
| [40] | Mondol, M., Chamon, A., Faiz, B., & Elahi, S. (2011). Seasonal variation of heavy metal concentrations in Water and plant samples around Tejgaon industrial Area of Bangladesh. Journal of Bangladesh academy of sciences, 35 (1), 19-41. |
| [41] | Sklensky, D. E. & Davies, P. J. 2011. Resource partitioning to male and female flowers of Spinacia oleracea L. in relation to whole-plant monocarpic senescence. Journal of Experimental Botany 62 (12): 4323-4336. |
| [42] | Munné-Bosch, S. (2008). Do perennials really senescence? Trends in Plant Science 13 (5): 216-220. |
| [43] | Okoli, E., & McEuen, A. (1986). Calcium-containing crystals in Telfairia hooker (Cucurbitaceae), New Phytol. 102, 199-207. |
| [44] | Kurien, A., & Radhamany P. (2007). Differential recognition of self-pollen and sterility in Hibiscus rosa-sinensis L. (Malvaceae), Adv. Pollen Spore Res., 25, 169-176. |
| [45] | Cronje, P., Barry, G., & Huysamer, M. (2011). Fruiting position during development of ‘Nules Clementine’mandarin affects the concentration of K, Mg and Ca in the flavedo. Scientia horticulturae, 130 (4), 829-837. |
| [46] | Jones, M. L. (2013). Mineral nutrient remobilization during corolla senescence in ethylene-sensitive and-insensitive flowers. AoB Plants, 5. |
| [47] | Van Doorn, W., & Woltering, E. (2008). Physiology and molecular biology of petal senescence. Journal of experimental botany, 59 (3), 453-480. |
| [48] | Ziblim, I. A., Timothy, K. A., & Phillip, A. (2012). Effects of Season on the Mineral (Potassium, Calcium, Phosphorus, Magnesium) Levels of Pennisetum Pedicellatum in Northern Ghana. Greener Journal of Agricultural Sciences, 2 (7), 329-333. |
| [49] | Khawas, P., Das, A., Sit, N., Badwaik, L., & Deka, S. (2014). Nutritional composition of culinary Musa ABB at different stages of development. American Journal of Food Science and Technology, 2 (3), 80-87. |
| [50] | Ncube, S., Saidi, P., & Halimani, T. (2015). Effect of stage of growth and processing methods on the nutritional content of Acacia angustissima leaf meal harvested for broiler feeding. Livest. Res. for Rural Develop, 27. |
| [51] | Pal, A., & Basistha, R. (2021). Effect of Maturity Stages on the Nutritional Content of Hygrophila spinosa and Chenopodium album Leaves. Journal of Scientific Research, 13 (3), 1011-1023. |
| [52] | Price, A. M., Aros Orellana, D., Salleh, F., Stevens, R., Acock, R., Buchanan-Wollaston, V., & Rogers, H. (2008). A comparison of leaf and petal senescence in wallflower reveals common and distinct patterns of gene expression and physiology. Plant Physiology, 147 (4), 1898-1912. |
| [53] | Thomas, H., & Ougham, H. (2015). Senescence and crop performance. In: Crop Physiology (pp. 223-249). Academic Press. |
| [54] | Zhao, G., Wei, S., Li, Y., Jeong, E., Kim, H., & Kim, J. (2020). Comparison of forage quality, productivity and β-carotene content according to maturity of forage rye (Secale cereale L.). Journal of the Korean Society of Grassland and Forage Science, 40 (3), 123-130. |
| [55] | Keskin, B., Akdeniz, H., Yilmaz, I., Turan, N. (2005). Yield and quality of forage corn (Zea mays L.) as influ-enced by cultivars and nitrogen rates. Journal of Agron-omy, 4: 138-41. |
| [56] | Stitt, M., & Krapp, A. (1999). The interaction between elevated carbon dioxide and nitrogen nutrition: the physiological and molecular background. Plant, Cell & Environment, 22 (6), 583-621. |
| [57] | Zhai, G., Shen, Y., Zhai, Y., Liu, X., & Jiang, H. (2008). Forage yield performance and nutritive value of selected wild soybean ecotypes. Canadian journal of plant science, 88 (3), 465-472. |
| [58] | Evitayani, E., Warly, L., Fariani, A., Ichinohe, T., & Fujihara, T. (2004). Seasonal changes in nutritive value of some grass species in West Sumatra, Indonesia. Asian-Australasian Journal of Animal Sciences, 17 (12), 1663-1668. |
| [59] | Sangu, G., & Nahonyo, C. (2021). Assessment of Seasonal Variations in Forage Quality in Saanane Island National Park, Mwanza, Tanzania. Tanzania Journal of Science, 47 (5), 1856-1869. |
| [60] | Biesiada, A., Kolota, E., & Adamczewska-sowinska, K. (2007). The effect of maturity stage on nutritional value of leek, zucchini and kohlrabi. Vegetable Crops Research Bulletin, 66, 39. |
| [61] | Laya, A., & Koubala, B. (2020). Changes in Vitamin E and β-Carotene Contents in Various Edible Cassava Leaves (Manihot esculenta Crantz) of Different Ages across Multiple Seasons. International Journal of Agronomy, 2020. |
| [62] | Mibei, E., Ambuko, J., Giovannoni, J., Onyango, A., & Owino, W. (2017). Carotenoid profiling of the leaves of selected African eggplant accessions subjected to drought stress. Food Science & Nutrition, 5 (1), 113-122. |
| [63] | Adelanwa, E., & Medugu, J. (2015). Variation in the nutrient composition of red and. Journal of Applied Agricultural Research, 7, 183-189. |
| [64] | Moore, K., & Jung, H. (2001). Lignin and fiber digestion. |
| [65] | Gurjeet, K., & Meenakshi, G. (2017). Effect of growth stages and fertility levels on growth, yield and quality of fodder oats (Avena sativa L.). Journal of Applied and Natural Science 9 (3): 1287 -1296. |
| [66] | Kökten, K., Kaplan, M., Hatipoğlu, R., Saruhan, V., & Çınar, S. (2012). Nutritive value of Mediterranean shrubs development. American Journal of Food Science and Technology, 2 (3), 80-87. |
| [67] | Bamishaiye, E., Olayemi, F., Awagu, E., & Bamshaiye, O. (2011). Proximate and phytochemical composition of Moringa oleifera leaves at three stages of maturation. Advance Journal of Food Science and Technology, 3 (4), 233-237. |
APA Style
Gitau Jane Wanjiku, Gathungu Geofrey Kingori, Kiramana James Kirimi. (2023). Effect of Harvesting Stage on Cowpea Leaf Nutrient Composition. Plant, 11(2), 50-59. https://doi.org/10.11648/j.plant.20231102.12
ACS Style
Gitau Jane Wanjiku; Gathungu Geofrey Kingori; Kiramana James Kirimi. Effect of Harvesting Stage on Cowpea Leaf Nutrient Composition. Plant. 2023, 11(2), 50-59. doi: 10.11648/j.plant.20231102.12
AMA Style
Gitau Jane Wanjiku, Gathungu Geofrey Kingori, Kiramana James Kirimi. Effect of Harvesting Stage on Cowpea Leaf Nutrient Composition. Plant. 2023;11(2):50-59. doi: 10.11648/j.plant.20231102.12
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Download@article{10.11648/j.plant.20231102.12,
author = {Gitau Jane Wanjiku and Gathungu Geofrey Kingori and Kiramana James Kirimi},
title = {Effect of Harvesting Stage on Cowpea Leaf Nutrient Composition},
journal = {Plant},
volume = {11},
number = {2},
pages = {50-59},
doi = {10.11648/j.plant.20231102.12},
url = {https://doi.org/10.11648/j.plant.20231102.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20231102.12},
abstract = {Cowpea leaves are enjoyed as vegetables in many parts of Africa as they contain a lot of antioxidants, micronutrients and nutraceuticals whose deficiency is prevalent among people in Sub-Saharan Africa. Cowpea leaves undergo several physiological and metabolic changes during their maturity stages which may affect their nutritional content. However, farmers lack knowledge on the best cowpea harvesting stage. This research therefore aimed at obtaining information on the right harvesting stage that would enhance cowpea utilization by farmers. Cowpeas variety M66 was planted in RCBD and the treatments which were replicated thrice included harvesting at 21, 35 and 49 DAS. Data was collected on chlorophyll content, iron, calcium, crude fibre, beta carotene, protein and moisture content. The data was subjected for variance using Statistical Analysis System 9.2 edition and significantly different means separated using LSD at 5%. The harvesting stage significantly (p ≤ 0.05) influenced the chlorophyll content with 49 DAS recording the highest content at 51.39 nm followed by 35 DAS with 41.87 nm and the least at 21 DAS with 22. 05 nm. The moisture content decreased with the stage of harvest with highest moisture content being observed at 21 DAS and the least at 49 DAS in both trials. The iron content of cowpea leaves was significantly (p ≤ 0.05) different at 49 DAS in both trials. The calcium content at 21 and 49 DAS in both trials was significantly (p ≤ 0.05) different. The protein content was significant (p ≤ 0.05) in all the stages of harvesting with the highest protein content in both trials being recorded at 21 DAS and the least being recorded at 49 DAS in both. Crude fibre content increased with the stage of harvesting in both trials. This research highlights the essence of harvesting cowpea leaves at the correct harvest stage for increased nutrient utilization.},
year = {2023}
}
TY - JOUR T1 - Effect of Harvesting Stage on Cowpea Leaf Nutrient Composition AU - Gitau Jane Wanjiku AU - Gathungu Geofrey Kingori AU - Kiramana James Kirimi Y1 - 2023/06/20 PY - 2023 N1 - https://doi.org/10.11648/j.plant.20231102.12 DO - 10.11648/j.plant.20231102.12 T2 - Plant JF - Plant JO - Plant SP - 50 EP - 59 PB - Science Publishing Group SN - 2331-0677 UR - https://doi.org/10.11648/j.plant.20231102.12 AB - Cowpea leaves are enjoyed as vegetables in many parts of Africa as they contain a lot of antioxidants, micronutrients and nutraceuticals whose deficiency is prevalent among people in Sub-Saharan Africa. Cowpea leaves undergo several physiological and metabolic changes during their maturity stages which may affect their nutritional content. However, farmers lack knowledge on the best cowpea harvesting stage. This research therefore aimed at obtaining information on the right harvesting stage that would enhance cowpea utilization by farmers. Cowpeas variety M66 was planted in RCBD and the treatments which were replicated thrice included harvesting at 21, 35 and 49 DAS. Data was collected on chlorophyll content, iron, calcium, crude fibre, beta carotene, protein and moisture content. The data was subjected for variance using Statistical Analysis System 9.2 edition and significantly different means separated using LSD at 5%. The harvesting stage significantly (p ≤ 0.05) influenced the chlorophyll content with 49 DAS recording the highest content at 51.39 nm followed by 35 DAS with 41.87 nm and the least at 21 DAS with 22. 05 nm. The moisture content decreased with the stage of harvest with highest moisture content being observed at 21 DAS and the least at 49 DAS in both trials. The iron content of cowpea leaves was significantly (p ≤ 0.05) different at 49 DAS in both trials. The calcium content at 21 and 49 DAS in both trials was significantly (p ≤ 0.05) different. The protein content was significant (p ≤ 0.05) in all the stages of harvesting with the highest protein content in both trials being recorded at 21 DAS and the least being recorded at 49 DAS in both. Crude fibre content increased with the stage of harvesting in both trials. This research highlights the essence of harvesting cowpea leaves at the correct harvest stage for increased nutrient utilization. VL - 11 IS - 2 ER -
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