Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch

Ryoko Shimada; Junichi Matsumoto; Mikiharu Doi; Kiyoshi Ebihara

doi:doi:10.11648/j.jfns.20190706.11

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Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara

Published in Journal of Food and Nutrition Sciences (Volume 7, Issue 6)

Received: 8 October 2019 Accepted: 25 October 2019 Published: 31 October 2019

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Abstract

It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.

Published in	Journal of Food and Nutrition Sciences (Volume 7, Issue 6)
DOI	10.11648/j.jfns.20190706.11
Page(s)	79-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Glucagon-Like Peptide-1, High Amylose Corn Starch, Resistant Protein, Short Chain Fatty Acids, Cecal Microbiota, Cecal Fermentation

References

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APA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. (2019). Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. Journal of Food and Nutrition Sciences, 7(6), 79-86. https://doi.org/10.11648/j.jfns.20190706.11

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ACS Style

Ryoko Shimada; Junichi Matsumoto; Mikiharu Doi; Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J. Food Nutr. Sci. 2019, 7(6), 79-86. doi: 10.11648/j.jfns.20190706.11

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AMA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J Food Nutr Sci. 2019;7(6):79-86. doi: 10.11648/j.jfns.20190706.11

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@article{10.11648/j.jfns.20190706.11,
  author = {Ryoko Shimada and Junichi Matsumoto and Mikiharu Doi and Kiyoshi Ebihara},
  title = {Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {7},
  number = {6},
  pages = {79-86},
  doi = {10.11648/j.jfns.20190706.11},
  url = {https://doi.org/10.11648/j.jfns.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20190706.11},
  abstract = {It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.},
 year = {2019}
}

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TY  - JOUR
T1  - Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch
AU  - Ryoko Shimada
AU  - Junichi Matsumoto
AU  - Mikiharu Doi
AU  - Kiyoshi Ebihara
Y1  - 2019/10/31
PY  - 2019
N1  - https://doi.org/10.11648/j.jfns.20190706.11
DO  - 10.11648/j.jfns.20190706.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 79
EP  - 86
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20190706.11
AB  - It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.
VL  - 7
IS  - 6
ER  -

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Author Information

Ryoko Shimada

School of Human Science and Environment, University of Hyogo, Himeji, Japan; Faculty of Health Sciences, Osaka Aoyama University, Minoh, Japan
Junichi Matsumoto

Marutomo Co. Ltd., Iyo, Japan
Mikiharu Doi

Marutomo Co. Ltd., Iyo, Japan
Kiyoshi Ebihara

Faculty of Health Sciences, Osaka Aoyama University, Minoh, Japan

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APA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. (2019). Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. Journal of Food and Nutrition Sciences, 7(6), 79-86. https://doi.org/10.11648/j.jfns.20190706.11

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ACS Style

Ryoko Shimada; Junichi Matsumoto; Mikiharu Doi; Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J. Food Nutr. Sci. 2019, 7(6), 79-86. doi: 10.11648/j.jfns.20190706.11

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AMA Style

Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara. Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch. J Food Nutr Sci. 2019;7(6):79-86. doi: 10.11648/j.jfns.20190706.11

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@article{10.11648/j.jfns.20190706.11,
  author = {Ryoko Shimada and Junichi Matsumoto and Mikiharu Doi and Kiyoshi Ebihara},
  title = {Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {7},
  number = {6},
  pages = {79-86},
  doi = {10.11648/j.jfns.20190706.11},
  url = {https://doi.org/10.11648/j.jfns.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20190706.11},
  abstract = {It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.},
 year = {2019}
}

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TY  - JOUR
T1  - Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch
AU  - Ryoko Shimada
AU  - Junichi Matsumoto
AU  - Mikiharu Doi
AU  - Kiyoshi Ebihara
Y1  - 2019/10/31
PY  - 2019
N1  - https://doi.org/10.11648/j.jfns.20190706.11
DO  - 10.11648/j.jfns.20190706.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 79
EP  - 86
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20190706.11
AB  - It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.
VL  - 7
IS  - 6
ER  -

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