Phenotypic Characteristics of <i>Klebsiella pneumoniae</i> Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon

Jean Teghonong; Cecile Okalla Ebongue; Callixte Yadufashije; Loick Kojom; Dieudonne Adiogo

doi:doi:10.11648/j.ejcbs.20200601.13

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Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Loick Kojom, Dieudonne Adiogo

Published in European Journal of Clinical and Biomedical Sciences (Volume 6, Issue 1)

Received: 8 January 2020 Accepted: 27 January 2020 Published: 11 February 2020

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Abstract

Background: the resistance of enterobacteriaceae to antibiotics is experiencing a worrying worldwide development with an increasing impact of β-lactamases. Klebsiella pneumoniae is one of the bacteria responsible for nosocomial infections. Hence the need to look for these enzymes in the hospitals of the Littoral region, Cameroon. Method: cross-sectional and descriptive study during 2016 and early 2017, isolates of intermediate sensitivity or resistant to third generation cephalosporins (C3G) collected in the laboratories of the Littoral referral hospitals and in Diagmed laboratory. Confirmation of the strains made by the API 20E™ gallery, the study of antibiotic sensitivity by the method of diffusion of discs on agar (Mueller Hinton). The double synergy test for the search of extended spectrum β-lactamases (ESBL), and confirmation by the Eurobio ™ kit (France). Results: a total of 412 strains of klebsiellasp (species) were isolated and 122 Klebsiella pneumoniae included. The majority age group is that ≤1 year with 26.0%, the germs were isolated in the urine at 54.6% and in neonatology and pediatrics at 35.8%. Klebsiella pneumoniae pneumoniae identified at 82.4%. The production of BLSE was 86.9%, against that of AmpC at 3.30%. The sensitivity to imipenem and amikacin was 96.2% and 98.1%, respectively. Conclusion: the study shows a high frequency of ESBL and a low presence of ampC in the Littoral region, Cameroon; practitioners must make a rational prescription from a correctly performed antibiogram.

Published in	European Journal of Clinical and Biomedical Sciences (Volume 6, Issue 1)
DOI	10.11648/j.ejcbs.20200601.13
Page(s)	9-13
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Klebsiella pneumoniae, Resistance, β-Lactamase

References

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[4]	El bouamri MC, Arsalane L, Kamouni Y, Berraha M, Zouhair S. Évolution récente du profil épidémiologique des entérobactéries uropathogènes productrices de β-lactamases à spectre élargi à Marrakech, Maroc. Elsevier Masson SAS. 2014; 24 (7): 451-455.
[5]	Camara M, Diop Ndiaye H, Ba-Diallo A, Karam F, Mbow M, Faye A et al. Epidémiologie des souches de Klebsiella pneumoniae productrices de β-lactamases à spectre élargi dans un hôpital universitaire au Sénégal, 2011. Rev. CAMES SANTE. 2013; 1 (2): 133–135.
[6]	Djuikoue IC, Woerther PL, Toukam M, Burdet C, Ruppé E, Gonsu KH et al. Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon. J Infect Dev Ctries. 2016 Oct 31; 10 (10): 1135-1139.
[7]	Gangoué-Piéboji J, Branka B, Koulla-Shiro S, Randegger C, Adiogo D, Ngassam P et al. Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae in Yaounde, Cameroon. J Clin Microbiology. 2005; 34 (7): 3273-3277.
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[11]	Flokas ME, Karanika S, Alevikazakos M, Mylonakis E. Prevalence of ESBL-Producing Enterobacteriaceae in Pediatric Bloodstream Infections: A Systematic Review and Meta-Analysis. PLOS ONE. 2017 Jan 31; 12 (1): e0171216. doi: 10.1371/journal.pone.0171216.
[12]	Farah A, Boutefnouchef N, Dekhil M, Bouzerna N. Klebsiella pneumoniae productrice de BLSE isolées dans les hôpitaux de la ville d’Annaba, Algérie. Scientific Study & Research. 2007; VIII (2): 1582-540X.
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APA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Loick Kojom, Dieudonne Adiogo. (2020). Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon. European Journal of Clinical and Biomedical Sciences, 6(1), 9-13. https://doi.org/10.11648/j.ejcbs.20200601.13

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ACS Style

Jean Teghonong; Cecile Okalla Ebongue; Callixte Yadufashije; Loick Kojom; Dieudonne Adiogo. Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon. Eur. J. Clin. Biomed. Sci. 2020, 6(1), 9-13. doi: 10.11648/j.ejcbs.20200601.13

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AMA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Loick Kojom, Dieudonne Adiogo. Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon. Eur J Clin Biomed Sci. 2020;6(1):9-13. doi: 10.11648/j.ejcbs.20200601.13

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@article{10.11648/j.ejcbs.20200601.13,
  author = {Jean Teghonong and Cecile Okalla Ebongue and Callixte Yadufashije and Loick Kojom and Dieudonne Adiogo},
  title = {Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {6},
  number = {1},
  pages = {9-13},
  doi = {10.11648/j.ejcbs.20200601.13},
  url = {https://doi.org/10.11648/j.ejcbs.20200601.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20200601.13},
  abstract = {Background: the resistance of enterobacteriaceae to antibiotics is experiencing a worrying worldwide development with an increasing impact of β-lactamases. Klebsiella pneumoniae is one of the bacteria responsible for nosocomial infections. Hence the need to look for these enzymes in the hospitals of the Littoral region, Cameroon. Method: cross-sectional and descriptive study during 2016 and early 2017, isolates of intermediate sensitivity or resistant to third generation cephalosporins (C3G) collected in the laboratories of the Littoral referral hospitals and in Diagmed laboratory. Confirmation of the strains made by the API 20E™ gallery, the study of antibiotic sensitivity by the method of diffusion of discs on agar (Mueller Hinton). The double synergy test for the search of extended spectrum β-lactamases (ESBL), and confirmation by the Eurobio ™ kit (France). Results: a total of 412 strains of klebsiellasp (species) were isolated and 122 Klebsiella pneumoniae included. The majority age group is that ≤1 year with 26.0%, the germs were isolated in the urine at 54.6% and in neonatology and pediatrics at 35.8%. Klebsiella pneumoniae pneumoniae identified at 82.4%. The production of BLSE was 86.9%, against that of AmpC at 3.30%. The sensitivity to imipenem and amikacin was 96.2% and 98.1%, respectively. Conclusion: the study shows a high frequency of ESBL and a low presence of ampC in the Littoral region, Cameroon; practitioners must make a rational prescription from a correctly performed antibiogram.},
 year = {2020}
}

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TY - JOUR
T1 - Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon
AU - Jean Teghonong
AU - Cecile Okalla Ebongue
AU - Callixte Yadufashije
AU - Loick Kojom
AU - Dieudonne Adiogo
Y1 - 2020/02/11
PY - 2020
N1 - https://doi.org/10.11648/j.ejcbs.20200601.13
DO - 10.11648/j.ejcbs.20200601.13
T2 - European Journal of Clinical and Biomedical Sciences
JF - European Journal of Clinical and Biomedical Sciences
JO - European Journal of Clinical and Biomedical Sciences
SP - 9
EP - 13
PB - Science Publishing Group
SN - 2575-5005
UR - https://doi.org/10.11648/j.ejcbs.20200601.13
AB - Background: the resistance of enterobacteriaceae to antibiotics is experiencing a worrying worldwide development with an increasing impact of β-lactamases. Klebsiella pneumoniae is one of the bacteria responsible for nosocomial infections. Hence the need to look for these enzymes in the hospitals of the Littoral region, Cameroon. Method: cross-sectional and descriptive study during 2016 and early 2017, isolates of intermediate sensitivity or resistant to third generation cephalosporins (C3G) collected in the laboratories of the Littoral referral hospitals and in Diagmed laboratory. Confirmation of the strains made by the API 20E™ gallery, the study of antibiotic sensitivity by the method of diffusion of discs on agar (Mueller Hinton). The double synergy test for the search of extended spectrum β-lactamases (ESBL), and confirmation by the Eurobio ™ kit (France). Results: a total of 412 strains of klebsiellasp (species) were isolated and 122 Klebsiella pneumoniae included. The majority age group is that ≤1 year with 26.0%, the germs were isolated in the urine at 54.6% and in neonatology and pediatrics at 35.8%. Klebsiella pneumoniae pneumoniae identified at 82.4%. The production of BLSE was 86.9%, against that of AmpC at 3.30%. The sensitivity to imipenem and amikacin was 96.2% and 98.1%, respectively. Conclusion: the study shows a high frequency of ESBL and a low presence of ampC in the Littoral region, Cameroon; practitioners must make a rational prescription from a correctly performed antibiogram.
VL - 6
IS - 1
ER -

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Author Information

Jean Teghonong

Department of Biological Sciences, Distant Production House University, Uvira, Democratic Republic of Congo; Diagmed Laboratory, Douala, Cameroon
Cecile Okalla Ebongue

Department of Biological Sciences, Douala University, Douala, Cameroon
Callixte Yadufashije

Ruhengeri-Institute of Applied Sciences, Musanze, Rwanda
Loick Kojom

Department of Animal Biology and Physiology, Douala University, Douala, Cameroon
Dieudonne Adiogo

Department of Biological Sciences, Douala University, Douala, Cameroon

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APA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Loick Kojom, Dieudonne Adiogo. (2020). Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon. European Journal of Clinical and Biomedical Sciences, 6(1), 9-13. https://doi.org/10.11648/j.ejcbs.20200601.13

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ACS Style

Jean Teghonong; Cecile Okalla Ebongue; Callixte Yadufashije; Loick Kojom; Dieudonne Adiogo. Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon. Eur. J. Clin. Biomed. Sci. 2020, 6(1), 9-13. doi: 10.11648/j.ejcbs.20200601.13

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AMA Style

Jean Teghonong, Cecile Okalla Ebongue, Callixte Yadufashije, Loick Kojom, Dieudonne Adiogo. Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon. Eur J Clin Biomed Sci. 2020;6(1):9-13. doi: 10.11648/j.ejcbs.20200601.13

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@article{10.11648/j.ejcbs.20200601.13,
  author = {Jean Teghonong and Cecile Okalla Ebongue and Callixte Yadufashije and Loick Kojom and Dieudonne Adiogo},
  title = {Phenotypic Characteristics of Klebsiella pneumoniae Extended Spectrum β-Lactamases Producers Isolated in Hospitals in the Littoral Region, Cameroon},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {6},
  number = {1},
  pages = {9-13},
  doi = {10.11648/j.ejcbs.20200601.13},
  url = {https://doi.org/10.11648/j.ejcbs.20200601.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20200601.13},
  abstract = {Background: the resistance of enterobacteriaceae to antibiotics is experiencing a worrying worldwide development with an increasing impact of β-lactamases. Klebsiella pneumoniae is one of the bacteria responsible for nosocomial infections. Hence the need to look for these enzymes in the hospitals of the Littoral region, Cameroon. Method: cross-sectional and descriptive study during 2016 and early 2017, isolates of intermediate sensitivity or resistant to third generation cephalosporins (C3G) collected in the laboratories of the Littoral referral hospitals and in Diagmed laboratory. Confirmation of the strains made by the API 20E™ gallery, the study of antibiotic sensitivity by the method of diffusion of discs on agar (Mueller Hinton). The double synergy test for the search of extended spectrum β-lactamases (ESBL), and confirmation by the Eurobio ™ kit (France). Results: a total of 412 strains of klebsiellasp (species) were isolated and 122 Klebsiella pneumoniae included. The majority age group is that ≤1 year with 26.0%, the germs were isolated in the urine at 54.6% and in neonatology and pediatrics at 35.8%. Klebsiella pneumoniae pneumoniae identified at 82.4%. The production of BLSE was 86.9%, against that of AmpC at 3.30%. The sensitivity to imipenem and amikacin was 96.2% and 98.1%, respectively. Conclusion: the study shows a high frequency of ESBL and a low presence of ampC in the Littoral region, Cameroon; practitioners must make a rational prescription from a correctly performed antibiogram.},
 year = {2020}
}

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