International Journal of Food Science and Biotechnology

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Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate

Received: Mar. 11, 2019    Accepted: May 15, 2019    Published: Oct. 24, 2019
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Abstract

Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.

DOI 10.11648/j.ijfsb.20190404.11
Published in International Journal of Food Science and Biotechnology ( Volume 4, Issue 4, December 2019 )
Page(s) 82-86
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Adaptation, Potassium Sorbate, Sodium Benzoate, E. coli, Morphological Changes

References
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    Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas. (2019). Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. International Journal of Food Science and Biotechnology, 4(4), 82-86. https://doi.org/10.11648/j.ijfsb.20190404.11

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    ACS Style

    Norma Angélica Santiesteban-López; Teresa Gladys Cerón-Carrillo; José Luis Carmona-Silva; Javier Castro-Rosas. Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. Int. J. Food Sci. Biotechnol. 2019, 4(4), 82-86. doi: 10.11648/j.ijfsb.20190404.11

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    AMA Style

    Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas. Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. Int J Food Sci Biotechnol. 2019;4(4):82-86. doi: 10.11648/j.ijfsb.20190404.11

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  • @article{10.11648/j.ijfsb.20190404.11,
      author = {Norma Angélica Santiesteban-López and Teresa Gladys Cerón-Carrillo and José Luis Carmona-Silva and Javier Castro-Rosas},
      title = {Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate},
      journal = {International Journal of Food Science and Biotechnology},
      volume = {4},
      number = {4},
      pages = {82-86},
      doi = {10.11648/j.ijfsb.20190404.11},
      url = {https://doi.org/10.11648/j.ijfsb.20190404.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijfsb.20190404.11},
      abstract = {Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.},
     year = {2019}
    }
    

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    T1  - Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate
    AU  - Norma Angélica Santiesteban-López
    AU  - Teresa Gladys Cerón-Carrillo
    AU  - José Luis Carmona-Silva
    AU  - Javier Castro-Rosas
    Y1  - 2019/10/24
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ijfsb.20190404.11
    DO  - 10.11648/j.ijfsb.20190404.11
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    JF  - International Journal of Food Science and Biotechnology
    JO  - International Journal of Food Science and Biotechnology
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    EP  - 86
    PB  - Science Publishing Group
    SN  - 2578-9643
    UR  - https://doi.org/10.11648/j.ijfsb.20190404.11
    AB  - Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.
    VL  - 4
    IS  - 4
    ER  - 

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Author Information
  • Faculty of Administration (Gastronomy). Benemérita Universidad Autónoma de Puebla, University City, Puebla, México

  • Faculty of Administration (Gastronomy). Benemérita Universidad Autónoma de Puebla, University City, Puebla, México

  • Faculty of Administration (Gastronomy). Benemérita Universidad Autónoma de Puebla, University City, Puebla, México

  • Academic Area of Chemistry, Institute of Basic Sciences and Engineering, Autonomous University of the State of Hidalgo, Hidalgo, México

  • Section