-
Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification
Vu Ngoc Boi
,
Nguyen Thi My Trang
,
Dang Xuan Cuong
,
Hoang Thai Ha
Issue: Volume 4, Issue 2, June 2020
Pages: 62-68
Received: Apr. 08, 2020
Accepted: Apr. 26, 2020
Published: May 19, 2020
DOI:
10.11648/j.wjfst.20200402.17
Downloads:
Views:
Abstract: The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and 1H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO4 equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO4 equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and 1H NMR spectrum showed phloroglucinol exist in two over fractions.
Abstract: The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotann...
Show More
-
Biophysical-chemistry Characterization of Alginate in Brown Algae Species Sargassum dupplicatum
Dang Xuan Cuong
,
Dang Thi Thanh Tuyen
,
Do Thuy Kieu
Issue: Volume 4, Issue 1, March 2020
Pages: 17-22
Received: Mar. 17, 2020
Accepted: Apr. 02, 2020
Published: Apr. 29, 2020
DOI:
10.11648/j.wjfst.20200401.13
Downloads:
Views:
Abstract: Alginate is a high-value biopolymer, exists in brown algae, and applied widely in numerous fields, for example, food, functional foods, and pharmaceutics. The study focused on the biophysical-chemistry characterization of alginate in brown algae species Sargassum dupplicatum grown commonly in Vietnam under the effect of NaCl, KCl, MgCl2, chitosan, carrageenan, and ethanol in different physical condition. Antioxidant activity of alginate and their compound was also studied. The results showed that Na_Alg, K_Alg, and Mg_Alg disperse the net style in the water. Ca_Alg, chitosan_Alg, and Carrageenan_Alg absorbed water and swelled for forming a sphere, yarn, and yarn in the water, respectively. Chitosan_Alg and carrageenan_Alg precipitated faster in ethanol, compared to Na_Alg, K_Alg, and Mg_Alg. 20% of ethanol did not cause the precipitation of Alginate salt, Chitosan_Alg, and carrageenan_Alg for 30 minutes. Alginate salt, Chitosan_Alg, and Carrageenan_Alg were full precipitated for 30 minutes when using ethanol concentration was more than 80%. Chitosan_Alg and Carrageenan_Alg occurred the precipitation in 20% of ethanol for 30 minutes. Total antioxidant activity and reducing power activity of chitosan_Alg got the highest value (a and b, respectively), compared to Carrageenan_Alg, Na_Alg, K_Alg, and Mg_Alg. The antioxidant activity difference in a group of Na_Alg, K_Alg, and Mg_Alg, and a group (Chitosan_Alg and Carrageenan_Alg) did not happen. The difference only occurred between the two groups.
Abstract: Alginate is a high-value biopolymer, exists in brown algae, and applied widely in numerous fields, for example, food, functional foods, and pharmaceutics. The study focused on the biophysical-chemistry characterization of alginate in brown algae species Sargassum dupplicatum grown commonly in Vietnam under the effect of NaCl, KCl, MgCl2, chitosan, ...
Show More
-
Effect of Ethanol on Physical Chemistry Characterization, Microorganism, and Toxicity of Carrageenan Extracted with the Assistant of Enzyme Viscozyme L
Bui Huy Chich
,
Do Van Ninh
,
Vu Ngoc Boi
,
Dang Xuan Cuong
Issue: Volume 4, Issue 1, March 2020
Pages: 23-30
Received: Mar. 17, 2020
Accepted: Apr. 03, 2020
Published: Apr. 29, 2020
DOI:
10.11648/j.wjfst.20200401.14
Downloads:
Views:
Abstract: Carrageenan is a biopolymer found in red algae with high potential in food, functional food, pharmaceutics, and cosmetics. The study focused on the effect of ethanol on physical chemistry characterization and microorganisms of carrageenan that extracted by the enzyme – assisted method and the purification by using ethanol. The results showed the moisture of carrageenan varied from 10.9 to 9.5% DW. After the impact of ethanol, the purification and physical (dispersal in water and rheological) characterization of carrageenan was higher than before the impact of ethanol. For example, dispersal in water, the viscosity of the solution, the solution strength (1.5% of carrageenan and 0.2% of potassium chloride), and carbohydrate content at 20°C corresponded to 1.06, 1.18, 1.07, and 1.11 times, compared to before the impact of ethanol. The content of ethanol-insolubility impurities, total ash, acid-solubility ash, acid-insolubility ash, total protein, sunphat content (SO42-), and lipid content was 43%, 94.6%, 42.9%, 44.44%, 3.9%, 97.2%, and none-detected in comparison to before the impact of ethanol. The content of lead, arsenic, cadmium, and mercury was 0.01, < 0.01, 0.05, and < 0.01 ppm, respectively. Total aerobic bacterial of carrageenan got the highest value of 2.1 x 102 cells/g. E. coli, coliforms, staphylococcus aureus, salmonella, and bacillus cereus did not occur in carrageenan.
Abstract: Carrageenan is a biopolymer found in red algae with high potential in food, functional food, pharmaceutics, and cosmetics. The study focused on the effect of ethanol on physical chemistry characterization and microorganisms of carrageenan that extracted by the enzyme – assisted method and the purification by using ethanol. The results showed the mo...
Show More
-
Laminarin (Beta-glucan) of Brown Algae Sargassum mcclurei: Extraction, Antioxidant Activity, Lipoxygenase Inhibition Activity, and Physicochemistry Properties
Issue: Volume 4, Issue 1, March 2020
Pages: 31-39
Received: Mar. 17, 2020
Accepted: Apr. 02, 2020
Published: Apr. 29, 2020
DOI:
10.11648/j.wjfst.20200401.15
Downloads:
Views:
Abstract: Laminarin is a storage glucan found in almost brown algae and possesses value bioactive. The study focused on the effect of the input factors of the extraction such as the temperature (30, 50, 70, and 90°C), the time (01, 02, 03, and 04 hours), the aqueous to algae ratio (20/1, 30/1, 40/1, and 50/1 (v/w)), and solvent pH (2, 7, and 9) on the function targets (laminarin content, total antioxidant activity, reducing power activity, and lipoxygenase inhibition activity), and the physical chemistry characterization of highest laminarin content was analyzed. The results showed the temperature, the time, the aqueous to algae, solvent pH affected on laminarin content, total antioxidant activity, reducing power activity, and lipoxygenase inhibition activity that got the highest value of 11.98±0.49 mg laminarin equivalent/g DW, 19.66±0.47 mg ascorbic acid equivalent/g DW, 15.55±0.61 mg FeSO4 equivalent/g DW, and 73.04±2.53 µM linoleic acid equivalent/g DW, respectively. The suitable condition of extraction was collected consisting of the temperature of 90°C for 2 hours with the solvent to algae ratio of 40/1 (v/w) in pH solvent of 7. The average molecular weight, viscosity, and sulfate content of laminarin was 505.18 kDa, 14,17±0.29 cPs, and 48.72%, respectively. FTIR spectrum exhibited function groups of laminarin composed of O-H, -CH2-, C=O, C-O, –C–H, -O-SO3-, and -CH=CH-(cis).
Abstract: Laminarin is a storage glucan found in almost brown algae and possesses value bioactive. The study focused on the effect of the input factors of the extraction such as the temperature (30, 50, 70, and 90°C), the time (01, 02, 03, and 04 hours), the aqueous to algae ratio (20/1, 30/1, 40/1, and 50/1 (v/w)), and solvent pH (2, 7, and 9) on the functi...
Show More
-
Oligosaccharide Chitosan: Viscosity, Molecular Weight, Antibacterial Activity, and Impact of γ Radiation
Vu Ngoc Boi
,
Nguyen Thi My Trang
,
Dang Xuan Cuong
,
Vu Thi Hoan
,
Le Hai
Issue: Volume 4, Issue 2, June 2020
Pages: 40-45
Received: Mar. 17, 2020
Accepted: Mar. 30, 2020
Published: Apr. 29, 2020
DOI:
10.11648/j.wjfst.20200402.14
Downloads:
Views:
Abstract: Chitosan is a bioactive polymer produced from shrimp and crab shells, etc. According to VASEP (Vietnam Association of Seafood Exporters and Producers), the production of raw shrimp cultured in Vietnam was about 800,000 tons in 2018. Therefore, the shrimp processing industry has generated about 320,000 tons of wastes, including heads and shells. If wastes are not utilized and managed in proper ways, it can lead to serious environmental problems. In our study, shrimp shells were used to produce chitosan and further obtained oligochitosan for application in food preservation. The cobalt-60 radiation technology has been used to segment chitosan into oligochitosan. The radiation dose applied to chitosan solution was in the range of 25 ÷ 50 kGy and in the range of 66 ÷ 166 kGy for chitosan flakes. The results showed that the chitosan solution had higher segmental efficiency compared to that of chitosan flakes. The antibacterial activities of oligosaccharide chitosan segmented from chitosan flakes were higher than those of oligosaccharide chitosan segmented from chitosan solution. The highest antibacterial activities were observed in the oligochitosan segmented from chitosan flakes at the radiation dose of 66 kGy for all tested bacteria: E. coli O157:H7, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis. In addition, oligochitosan segmented from chitosan flakes at the radiation dose of 66 kGy had higher antibacterial activities on bacteria gram (-) than bacteria gram (+). The strongest antibacterial activities on L. monocytogenes and B. subtilis at the concentration of 0.3125%.
Abstract: Chitosan is a bioactive polymer produced from shrimp and crab shells, etc. According to VASEP (Vietnam Association of Seafood Exporters and Producers), the production of raw shrimp cultured in Vietnam was about 800,000 tons in 2018. Therefore, the shrimp processing industry has generated about 320,000 tons of wastes, including heads and shells. If ...
Show More
-
Effect of Drying Temperatures and Aids on Polyphenol Content, Antioxidant Activity, and β Glucosidase Enzyme Inhibition Activity of Powder Stylissa flexibilis
Dang Xuan Cuong
,
Nguyen Thi Phuong Hien
Issue: Volume 4, Issue 2, June 2020
Pages: 46-52
Received: Mar. 17, 2020
Accepted: Apr. 02, 2020
Published: Apr. 29, 2020
DOI:
10.11648/j.wjfst.20200402.15
Downloads:
Views:
Abstract: Sponge Stylissa flexibilis is Porifera belong to a medicine animal group in the Vietnam sea, contains numerous valuable bioactive substances. The study focused on the impact of spray-drying input temperatures (90°C, 100°C, 110°C, and 120°C) and aids (Maltodextrin, saccharose: maltodextrin mixture (1:1, w/w and 1:2, w/w)) on the physical-chemical properties (moisture content, solubility, and polyphenol content), antioxidant activities (mechanism Mo6+ and Fe3+), and inhibition enzyme β Glucosidase of sponge extract powder. The sponge was collected from the sponge in May 2017 in the Ninh Thuan sea. The results showed the polyphenol content, total antioxidant activity, reducing power activity, and β Glucosidase enzyme inhibition activity was affected by the temperature and aids of the drying process and got the highest value of 77.692 ± 2.025 mg phloroglucinol equivalent/100g DW, 98.526 ± 1.997 mg ascorbic acid equivalent/100g DW, 28.945 ± 0.754 mg FeSO4 equivalent/100g DW, and 75.38 ± 2.516/100 μg/ml was at 90°C, respectively, compared to the others. The moisture of powder was a negative-correlation to the increase of drying temperature. All the powder dissolved fully in the water. Polyphenol content and bioactivities (antioxidant activity, β Glucosidase enzyme inhibition activity) was a strong correlation with each other (R2>0.8) and a positive-correlation to drying temperature. The suitable condition for drying of active polyphenol powder was at 90°C and 10% of maltodextrin. Active polyphenol powder can be useful for applicating into functional foods and pharmaceutics.
Abstract: Sponge Stylissa flexibilis is Porifera belong to a medicine animal group in the Vietnam sea, contains numerous valuable bioactive substances. The study focused on the impact of spray-drying input temperatures (90°C, 100°C, 110°C, and 120°C) and aids (Maltodextrin, saccharose: maltodextrin mixture (1:1, w/w and 1:2, w/w)) on the physical-chemical pr...
Show More