NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon

Marie-Claire Vernyuy Fonyuy; Calvino Fomboh Tah; Akindeh Mbuh Nji; Isabelle Mboutchuin Kamdem Winnie; Jean Paul Kengne Chedjou; Magellan Guewo Fokeng; Aristid Herve Ekollo Mbange; Wilfried Olivier Ngandjeu Tchamdjeu; Lesley Ngum Ngum; Peter Thelma Ngwa Niba; Rodrigue Essomba Foe; Cedric Hermann Dongmo; Carine Nguefeu Nkenfou-Tchinda; Rhoda Bongshe Laban; Yusinyu Eugenie Mumah; Eugene Sobngwi; Jean Claude Mbanya; Wilfred Fon Mbacham

doi:doi:10.11648/j.bmb.20230803.11

Research Article |

NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon

Marie-Claire Vernyuy Fonyuy, Calvino Fomboh Tah, Akindeh Mbuh Nji, Isabelle Mboutchuin Kamdem Winnie, Jean Paul Kengne Chedjou, Magellan Guewo Fokeng, Aristid Herve Ekollo Mbange, Wilfried Olivier Ngandjeu Tchamdjeu, Lesley Ngum Ngum, Peter Thelma Ngwa Niba, Rodrigue Essomba Foe, Cedric Hermann Dongmo, Carine Nguefeu Nkenfou-Tchinda, Rhoda Bongshe Laban, Yusinyu Eugenie Mumah, Eugene Sobngwi, Jean Claude Mbanya, Wilfred Fon Mbacham^*

Published in Biochemistry and Molecular Biology (Volume 8, Issue 3)

Received: 27 October 2023 Accepted: 14 November 2023 Published: 30 November 2023

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Abstract

Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X²) was used to establish associations. A P-value of <0.05 was considered significant. Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.

Published in	Biochemistry and Molecular Biology (Volume 8, Issue 3)
DOI	10.11648/j.bmb.20230803.11
Page(s)	37-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

NLRP3, Gene Polymorphism, Type 2 Diabetes Mellitus, Malaria, Co-morbidity, Susceptibility

References

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Fonyuy, M. V., Tah, C. F., Nji, A. M., Winnie, I. M. K., Chedjou, J. P. K., et al. (2023). NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochemistry and Molecular Biology, 8(3), 37-44. https://doi.org/10.11648/j.bmb.20230803.11

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Fonyuy, M. V.; Tah, C. F.; Nji, A. M.; Winnie, I. M. K.; Chedjou, J. P. K., et al. NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochem. Mol. Biol. 2023, 8(3), 37-44. doi: 10.11648/j.bmb.20230803.11

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Fonyuy MV, Tah CF, Nji AM, Winnie IMK, Chedjou JPK, et al. NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochem Mol Biol. 2023;8(3):37-44. doi: 10.11648/j.bmb.20230803.11

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@article{10.11648/j.bmb.20230803.11,
  author = {Marie-Claire Vernyuy Fonyuy and Calvino Fomboh Tah and Akindeh Mbuh Nji and Isabelle Mboutchuin Kamdem Winnie and Jean Paul Kengne Chedjou and Magellan Guewo Fokeng and Aristid Herve Ekollo Mbange and Wilfried Olivier Ngandjeu Tchamdjeu and Lesley Ngum Ngum and Peter Thelma Ngwa Niba and Rodrigue Essomba Foe and Cedric Hermann Dongmo and Carine Nguefeu Nkenfou-Tchinda and Rhoda Bongshe Laban and Yusinyu Eugenie Mumah and Eugene Sobngwi and Jean Claude Mbanya and Wilfred Fon Mbacham},
  title = {NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon},
  journal = {Biochemistry and Molecular Biology},
  volume = {8},
  number = {3},
  pages = {37-44},
  doi = {10.11648/j.bmb.20230803.11},
  url = {https://doi.org/10.11648/j.bmb.20230803.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20230803.11},
  abstract = {Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.
},
 year = {2023}
}

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TY  - JOUR
T1  - NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon
AU  - Marie-Claire Vernyuy Fonyuy
AU  - Calvino Fomboh Tah
AU  - Akindeh Mbuh Nji
AU  - Isabelle Mboutchuin Kamdem Winnie
AU  - Jean Paul Kengne Chedjou
AU  - Magellan Guewo Fokeng
AU  - Aristid Herve Ekollo Mbange
AU  - Wilfried Olivier Ngandjeu Tchamdjeu
AU  - Lesley Ngum Ngum
AU  - Peter Thelma Ngwa Niba
AU  - Rodrigue Essomba Foe
AU  - Cedric Hermann Dongmo
AU  - Carine Nguefeu Nkenfou-Tchinda
AU  - Rhoda Bongshe Laban
AU  - Yusinyu Eugenie Mumah
AU  - Eugene Sobngwi
AU  - Jean Claude Mbanya
AU  - Wilfred Fon Mbacham
Y1  - 2023/11/30
PY  - 2023
N1  - https://doi.org/10.11648/j.bmb.20230803.11
DO  - 10.11648/j.bmb.20230803.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20230803.11
AB  - Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.

VL  - 8
IS  - 3
ER  -

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Marie-Claire Vernyuy Fonyuy

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Strateos, 458 Carlton Court, South San Francisco, California, United States of America
Calvino Fomboh Tah

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Fobang Institutes for Innovations in Science and Technology, Simbock, Yaounde, Cameroon
Akindeh Mbuh Nji

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Fobang Institutes for Innovations in Science and Technology, Simbock, Yaounde, Cameroon
Isabelle Mboutchuin Kamdem Winnie

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon
Jean Paul Kengne Chedjou

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Buea, Buea, Cameroon
Magellan Guewo Fokeng

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon
Aristid Herve Ekollo Mbange

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; University Institute of Technologies, University of Ngaoundere, Ngaoundere, Cameroon
Wilfried Olivier Ngandjeu Tchamdjeu

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Lesley Ngum Ngum

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon; Institute of Medicine and Medicinal Plants Studies, Yaounde, Cameroon
Peter Thelma Ngwa Niba

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Rodrigue Essomba Foe

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon
Cedric Hermann Dongmo

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Carine Nguefeu Nkenfou-Tchinda

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Rhoda Bongshe Laban

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Yusinyu Eugenie Mumah

Gandhi Institute of Technology and Management (GITAM) University, Visakhapatnam, India
Eugene Sobngwi

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Jean Claude Mbanya

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon
Wilfred Fon Mbacham

Biotechnology Center, University of Yaounde I, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaounde I, Yaounde, Cameroon; Fobang Institutes for Innovations in Science and Technology, Simbock, Yaounde, Cameroon; Department of Biochemistry, Physiology and Pharmacology, Faculty of Medicine and Biomedical Science, University of Yaounde I, Yaounde, Cameroon

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Fonyuy, M. V., Tah, C. F., Nji, A. M., Winnie, I. M. K., Chedjou, J. P. K., et al. (2023). NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochemistry and Molecular Biology, 8(3), 37-44. https://doi.org/10.11648/j.bmb.20230803.11

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ACS Style

Fonyuy, M. V.; Tah, C. F.; Nji, A. M.; Winnie, I. M. K.; Chedjou, J. P. K., et al. NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochem. Mol. Biol. 2023, 8(3), 37-44. doi: 10.11648/j.bmb.20230803.11

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AMA Style

Fonyuy MV, Tah CF, Nji AM, Winnie IMK, Chedjou JPK, et al. NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon. Biochem Mol Biol. 2023;8(3):37-44. doi: 10.11648/j.bmb.20230803.11

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@article{10.11648/j.bmb.20230803.11,
  author = {Marie-Claire Vernyuy Fonyuy and Calvino Fomboh Tah and Akindeh Mbuh Nji and Isabelle Mboutchuin Kamdem Winnie and Jean Paul Kengne Chedjou and Magellan Guewo Fokeng and Aristid Herve Ekollo Mbange and Wilfried Olivier Ngandjeu Tchamdjeu and Lesley Ngum Ngum and Peter Thelma Ngwa Niba and Rodrigue Essomba Foe and Cedric Hermann Dongmo and Carine Nguefeu Nkenfou-Tchinda and Rhoda Bongshe Laban and Yusinyu Eugenie Mumah and Eugene Sobngwi and Jean Claude Mbanya and Wilfred Fon Mbacham},
  title = {NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon},
  journal = {Biochemistry and Molecular Biology},
  volume = {8},
  number = {3},
  pages = {37-44},
  doi = {10.11648/j.bmb.20230803.11},
  url = {https://doi.org/10.11648/j.bmb.20230803.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20230803.11},
  abstract = {Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.
},
 year = {2023}
}

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TY  - JOUR
T1  - NLRP3 Gene Polymorphisms and Association with Type 2 Diabetes mellitus and Malaria Co-morbidity in Yaounde, Cameroon
AU  - Marie-Claire Vernyuy Fonyuy
AU  - Calvino Fomboh Tah
AU  - Akindeh Mbuh Nji
AU  - Isabelle Mboutchuin Kamdem Winnie
AU  - Jean Paul Kengne Chedjou
AU  - Magellan Guewo Fokeng
AU  - Aristid Herve Ekollo Mbange
AU  - Wilfried Olivier Ngandjeu Tchamdjeu
AU  - Lesley Ngum Ngum
AU  - Peter Thelma Ngwa Niba
AU  - Rodrigue Essomba Foe
AU  - Cedric Hermann Dongmo
AU  - Carine Nguefeu Nkenfou-Tchinda
AU  - Rhoda Bongshe Laban
AU  - Yusinyu Eugenie Mumah
AU  - Eugene Sobngwi
AU  - Jean Claude Mbanya
AU  - Wilfred Fon Mbacham
Y1  - 2023/11/30
PY  - 2023
N1  - https://doi.org/10.11648/j.bmb.20230803.11
DO  - 10.11648/j.bmb.20230803.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20230803.11
AB  - Background: Diabetes is increasingly prevalent in malaria endemic settings like Cameroon thus contributing to a double burden in the management of these inflammatory diseases. Studies have shown that NLRP3 inflammasome plays a key role in type-2 diabetes (T2DM) and malaria induced inflammation. However, the hypothesis that the Single Nucleotides Polymorphisms (rs10754558 and rs4612666) in the NLRP3 gene could be associated with T2DM and malaria comorbidity is relatively new. This study aimed at determining the association between NLRP3 rs10754558 and rs4612666 Single Nucleotide Polymorphisms with susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in Yaoundé, Cameroon. Methods: A case-control study was performed on 100 conveniently collected blood samples, spotted on Whartman N° 3 filter paper from which DNA was extracted by the chelex-100 boiling method. Nested-PCR was used to confirm the presence of malaria and speciate Plasmodium spp. Genotyping of the NLRP3 gene SNPs was performed using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi-square test (X2) was used to establish associations. A P-value of Results: The mean age of the study population was 55±12.38 years. Eighty-eight (88) participants were diagnosed with T2DM, whereof 7 (7.95%) were ascertained by nested-PCR to harbour malaria; P. falciparum being the dominant circulating species. The most predominant genotype and allele for rs10754558 and rs4612666, was the heterozygous genotype GC and wildtype allele G (52.00%, 69.00%), and the homozygous mutant genotype CC and mutant allele C (63.00%, 76.50%) respectively. No statistical significance was found between the comorbid group and diabetes positive /malaria negative (D+M-) control group for the rs10754558 and rs4612666 SNPs. Statistical significance was found between the comorbid group and the diabetes negative/ malaria positive (D-M+) control group for the rs4612666 SNP. Individuals possessing the CC genotypes were 8 times more susceptible to diabetes and malaria comorbidity (OR=8.000, P=0.043), whereas individuals possessing the TC genotype were less susceptible (OR=0.079, P=0.030). Conclusion: An association was found between the NLRP3 rs4612666 SNP and susceptibility to Type 2 Diabetes mellitus and malaria comorbidity in our study.

VL  - 8
IS  - 3
ER  -

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