Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells

Kristin Zindel; Sarah Whiteman; Kurstin Wiewel; Anne Turner; Meghan McGivern; Suzanne Stapleton; Marti Jett; Chanaka Mendis

doi:doi:10.11648/j.bmb.20240901.11

Research Article |

Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells

Kristin Zindel, Sarah Whiteman, Kurstin Wiewel, Anne Turner, Meghan McGivern, Suzanne Stapleton, Marti Jett, Chanaka Mendis^*

Published in Biochemistry and Molecular Biology (Volume 9, Issue 1)

Received: 28 November 2023 Accepted: 23 December 2023 Published: 8 January 2024

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Abstract

Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.

Published in	Biochemistry and Molecular Biology (Volume 9, Issue 1)
DOI	10.11648/j.bmb.20240901.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lipopolysaccharide, Signaling Activities, cJun N-terminal Kinase, Gene Expression

References

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APA Style

Zindel, K., Whiteman, S., Wiewel, K., Turner, A., McGivern, M., et al. (2024). Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochemistry and Molecular Biology, 9(1), 1-6. https://doi.org/10.11648/j.bmb.20240901.11

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ACS Style

Zindel, K.; Whiteman, S.; Wiewel, K.; Turner, A.; McGivern, M., et al. Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochem. Mol. Biol. 2024, 9(1), 1-6. doi: 10.11648/j.bmb.20240901.11

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AMA Style

Zindel K, Whiteman S, Wiewel K, Turner A, McGivern M, et al. Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochem Mol Biol. 2024;9(1):1-6. doi: 10.11648/j.bmb.20240901.11

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@article{10.11648/j.bmb.20240901.11,
  author = {Kristin Zindel and Sarah Whiteman and Kurstin Wiewel and Anne Turner and Meghan McGivern and Suzanne Stapleton and Marti Jett and Chanaka Mendis},
  title = {Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells},
  journal = {Biochemistry and Molecular Biology},
  volume = {9},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.bmb.20240901.11},
  url = {https://doi.org/10.11648/j.bmb.20240901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20240901.11},
  abstract = {Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.
},
 year = {2024}
}

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TY  - JOUR
T1  - Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells
AU  - Kristin Zindel
AU  - Sarah Whiteman
AU  - Kurstin Wiewel
AU  - Anne Turner
AU  - Meghan McGivern
AU  - Suzanne Stapleton
AU  - Marti Jett
AU  - Chanaka Mendis
Y1  - 2024/01/08
PY  - 2024
N1  - https://doi.org/10.11648/j.bmb.20240901.11
DO  - 10.11648/j.bmb.20240901.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
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EP  - 6
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20240901.11
AB  - Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.

VL  - 9
IS  - 1
ER  -

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Author Information

Kristin Zindel

Department of Chemistry, UW-Platteville, Platteville, United States of America
Sarah Whiteman

Department of Chemistry, UW-Platteville, Platteville, United States of America
Kurstin Wiewel

Department of Chemistry, UW-Platteville, Platteville, United States of America
Anne Turner

Department of Chemistry, UW-Platteville, Platteville, United States of America
Meghan McGivern

Department of Chemistry, UW-Platteville, Platteville, United States of America
Suzanne Stapleton

Department of Chemistry, UW-Platteville, Platteville, United States of America
Marti Jett

Department of Molecular Pathology, Walter Reed Army Institute of Research, Silver Spring, United State of America
Chanaka Mendis

Department of Chemistry, UW-Platteville, Platteville, United States of America

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Plain Text BibTeX RIS

APA Style

Zindel, K., Whiteman, S., Wiewel, K., Turner, A., McGivern, M., et al. (2024). Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochemistry and Molecular Biology, 9(1), 1-6. https://doi.org/10.11648/j.bmb.20240901.11

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ACS Style

Zindel, K.; Whiteman, S.; Wiewel, K.; Turner, A.; McGivern, M., et al. Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochem. Mol. Biol. 2024, 9(1), 1-6. doi: 10.11648/j.bmb.20240901.11

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AMA Style

Zindel K, Whiteman S, Wiewel K, Turner A, McGivern M, et al. Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells. Biochem Mol Biol. 2024;9(1):1-6. doi: 10.11648/j.bmb.20240901.11

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@article{10.11648/j.bmb.20240901.11,
  author = {Kristin Zindel and Sarah Whiteman and Kurstin Wiewel and Anne Turner and Meghan McGivern and Suzanne Stapleton and Marti Jett and Chanaka Mendis},
  title = {Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells},
  journal = {Biochemistry and Molecular Biology},
  volume = {9},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.bmb.20240901.11},
  url = {https://doi.org/10.11648/j.bmb.20240901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20240901.11},
  abstract = {Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.
},
 year = {2024}
}

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TY  - JOUR
T1  - Evaluating Gene Expression and Signaling Activities in Lipopolysaccharide Induced Human Peripheral Blood Mononuclear Cells
AU  - Kristin Zindel
AU  - Sarah Whiteman
AU  - Kurstin Wiewel
AU  - Anne Turner
AU  - Meghan McGivern
AU  - Suzanne Stapleton
AU  - Marti Jett
AU  - Chanaka Mendis
Y1  - 2024/01/08
PY  - 2024
N1  - https://doi.org/10.11648/j.bmb.20240901.11
DO  - 10.11648/j.bmb.20240901.11
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20240901.11
AB  - Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro-inflammatory cytokines in multiple cell types that ultimately could lead to septic shock in humans. This study is aimed at identifying a set of functionally relevant genes induced by LPS and evaluating the effect of signal transduction pathway inter-connector, cJun N-terminal Kinase (JNK), on LPS induced gene and protein expression in human Peripheral Blood Mononuclear Cells (hPBMCs). A specific gene expression pattern induced by LPS was identified through microarray analysis and was confirmed in a time dependent manner by Reverse Transcription Polymerase Chain reaction (RT-PCR). In the presence of JNK specific inhibitor also known as anthrapyrazolone inhibitor and/or SP600125 both analyzed gene and protein expression that somewhat explained LPS induced cellular activities were altered. We believe the study will allow us to not only identify a set of functionally relevant genes induced by LPS but also better understand the role played by the complex interactions of multiple signal transduction pathways induced by LPS.

VL  - 9
IS  - 1
ER  -

Copy | Download