Research Article
Candida Species Isolated in Female Patients of Reproductive Age with Vaginal Candidiasis in Gualeguaychú, Entre Ríos, Argentina
Pérez Duarte Iván Rodrigo*
,
Razetto Georgina,
Leiva Silvina Érica,
Torres Luciano,
Juárez María Josefina
Issue:
Volume 13, Issue 2, December 2025
Pages:
30-36
Received:
18 September 2024
Accepted:
22 October 2024
Published:
26 August 2025
Abstract: Objective: To identify the Candida species isolated in female patients of reproductive age with vaginal candidiasis. To determine the antifungal (ATF) sensitivity profile of the isolated Candida species. Materials and methods: Cross-sectional descriptive study, in which 124 Candida spp strains from vaginal discharge samples were isolated in Sabouraud medium supplemented with glucose. CHRO Magar Candida was used for species identification and complementary tests such as germ tube, chlamydoconidia development and investigation of trehalose assimilation were performed. In vitro sensitivity was investigated by diffusion method with ATF discs fluconazole (FLU), itraconazole (ITRA) and amphotericin B (AMB). Results: C. albicans was observed in 85.5% of the isolates followed by C. glabrata with 7.3%, C. krusei 4%, C. tropicalis 2.4% and other Candida species. For FLU, 0.9% of C. albicans, 11.1% of C. glabrata and 100% of C. krusei isolates showed resistance. For ITRA, 17% of C. albicans isolates, 55.6% of C. glabrata and 100% of C. krusei were resistant. There were no isolates resistant to AMB. Conclusions: Vaginal candidiasis continues to present Candida albicans as the main etiological agent, which is widely sensitive to ATFs. C. glabrata and C. krusei species show increased resistance to azoles. The results obtained ratify the growing need for Candida species identification tests and determination of in vitro sensitivity to ATFs in order to guide the treatment of vaginal candidiasis towards therapeutic success.
Abstract: Objective: To identify the Candida species isolated in female patients of reproductive age with vaginal candidiasis. To determine the antifungal (ATF) sensitivity profile of the isolated Candida species. Materials and methods: Cross-sectional descriptive study, in which 124 Candida spp strains from vaginal discharge samples were isolated in Saboura...
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Research Article
Control of Genetic Purity of Parental Lines of Sunflower by Allelic Variants of Electrophoretic Spectra of Seed Storage Proteins
Igor Aksyonov*
Issue:
Volume 13, Issue 2, December 2025
Pages:
37-44
Received:
18 May 2025
Accepted:
5 June 2025
Published:
8 September 2025
Abstract: The efficiency of sunflower hybrid breeding is determined by the genetic purity of the parent lines. Traditionally, plant selection in the process of seed production of the obtained lines is performed by morphological traits. This does not always ensure high typicality of the lines. Evaluation of plants of sunflower lines only by morphological traits is not always effective, and in some cases leads to a decrease in the typicality level of both lines and hybrid combinations. The studies have established and identified allelic variants of electrophoretic spectra of seed storage proteins. Simultaneous evaluation and selection of plants by electrophoretic spectra of storage proteins and by morphological traits of plants ensured an increase and preservation of the genetic homogeneity of the maternal line GE 106. The using of allelic variants of electrophoretic spectra of proteins in plant selection ensured the genetic purity of the sterile analog CYTSrfrf (genotype has sterile cytoplasm, genes for restoration of polly fertility in homozygous recessive state) of the line at the level of 98.7%, the fertile analog CYTNrfrf of the line at the level of 98.6% (method of soil control). With this selection method, the analysis of plants by allelic variants of protein spectra increased the indicators of genetic purity of the maternal line components. The level of genetic purity of the sterile and fertile analogue, determined by allelic variants of the protein spectra of seeds, in the sterile analog CYTSrfrf and the fixer of sterility CYTNrfrf of the line was 99.6% and 99.8%. Establishing typicality by protein spectra in some cases helps to increase the indicators of genetic purity of lines, since it does not allow identifying atypical plants by height.
Abstract: The efficiency of sunflower hybrid breeding is determined by the genetic purity of the parent lines. Traditionally, plant selection in the process of seed production of the obtained lines is performed by morphological traits. This does not always ensure high typicality of the lines. Evaluation of plants of sunflower lines only by morphological trai...
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