Research Article
Inhibition of TRF1 Can Accelerate Aging and Induce Autophagy Through the P53-SIRT6 Pathway in Glioblastoma Multiforme
Ziyang Nie,
Siqi Liu,
Xinyu Huang,
Xin Geng*
Issue:
Volume 11, Issue 2, June 2025
Pages:
11-27
Received:
17 April 2025
Accepted:
28 April 2025
Published:
29 May 2025
Abstract: Glioblastoma (GBM) is a kind of intractable brain tumor. The effect of surgical treatment, radiotherapy and chemotherapy is not ideal. TRF1 is one of the important components of shelterin complex, which plays an important role in human telomere protection. Previous studies have reported that inhibition of TRF1 expression can inhibit the growth and proliferation of GBM without causing serious physiological dysfunction. However, the specific mechanism of inhibition of GBM growth and proliferation caused by decreased TRF1 expression has not been fully elucidated. To further elucidate this mechanism, we knockdown TRF1 by siRNA. We detected the levels of cell senescence, autophagy through biological experiments. It has been found that the knockdown of TRF1 can cause significant increase in the aging, autophagy of GBM. In addition, SIRT-6 is a NAD+- dependent deacetylase. Previous studies have reported that SIRT-6 can maintain the stability of telomere function. Moreover, telomere dysfunction can cause the decrease of SIRT-6 expression. Therefore, we want to study the effect of SIRT-6 expression level on TRF1 knockdown induced aging, autophagy in GBM. The experimental results showed that the knockdown of TRF1 caused the decrease of SIRT-6 expression level, and the increase of SIRT-6 expression level could inhibit the aging, autophagy caused by TRF1 knockdown. This study provides a new direction for the treatment of GBM.
Abstract: Glioblastoma (GBM) is a kind of intractable brain tumor. The effect of surgical treatment, radiotherapy and chemotherapy is not ideal. TRF1 is one of the important components of shelterin complex, which plays an important role in human telomere protection. Previous studies have reported that inhibition of TRF1 expression can inhibit the growth and ...
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Research Article
Magnitude and Determinants of Surgical Site Infections Following Cesarean Section: A Retrospective Multi-Hospital Study in Addis Ababa, Ethiopia, 2024
Abdurehman Seid Mohammed*
,
Abate Assefa Kassaw,
Rejeb Nesru Kemal
Issue:
Volume 11, Issue 2, June 2025
Pages:
28-39
Received:
1 January 2025
Accepted:
8 July 2025
Published:
18 August 2025
Abstract: Background: Over the past three decades, infections following cesarean sections have increased markedly, with surgical site infections (SSIs) emerging as a leading cause of maternal morbidity and mortality. This issue is especially critical in low- and middle-income countries, including Ethiopia, where reliable data on the magnitude and determinants of post-cesarean infections remain limited. These infections contribute to prolonged hospital stays, higher healthcare costs, and elevated risks for both mothers and newborns. Objective: This study aimed to assess the prevalence of post-cesarean section infections and identify associated risk factors in selected hospitals in Addis Ababa, Ethiopia, in 2024. Methods: An institution-based retrospective cross-sectional study was conducted in selected hospitals between June 10 and August 30, 2024. A total of 472 medical records of women who underwent cesarean sections from January 1, 2019, through December 31, 2023, were reviewed using a systematic random sampling method. Hospitals were selected using simple random sampling. Data were entered and cleaned using Epi Info version 7.2.1.0 and analyzed with SPSS version 26. Binary logistic regression was employed to identify factors associated with post-cesarean infections. Adjusted odds ratios (AORs) with 95% confidence intervals (CIs) were calculated, and statistical significance was determined at a p-value < 0.05. Results: The prevalence of post-cesarean section infection was 17%. Key factors significantly associated with increased risk included: presence of clinical signs of infection (AOR = 4.37, 95% CI: 3.25-6.22), underlying co-morbid conditions (AOR = 4.72, 95% CI: 3.34-6.23), and operation duration longer than 30 minutes (AOR = 3.43, 95% CI: 2.32-4.43). Conclusion and Recommendation: The prevalence of post-cesarean infections in Addis Ababa was alarmingly high. Major risk factors included signs of infection, co-morbid illnesses, prolonged labor (>24 hours), previous cesarean deliveries, and extended operative time. To reduce infection rates, hospitals should enforce strict infection prevention protocols and maintain high standards of surgical practice.
Abstract: Background: Over the past three decades, infections following cesarean sections have increased markedly, with surgical site infections (SSIs) emerging as a leading cause of maternal morbidity and mortality. This issue is especially critical in low- and middle-income countries, including Ethiopia, where reliable data on the magnitude and determinant...
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